多动小鼠脑内乙酰胆碱和八肽胆囊收缩素的改变

摘要

目的：了解多动小鼠脑内乙酰胆碱(Ach)和八肽胆囊收缩素(cck—8)的改变，探讨多动症的发病机理。方法：制备多动小鼠，采用放射免疫法测定其脑组织匀浆中的Ach和cck—8的含量。结果：腹腔注射东莨菪碱(3mg/kg)30min后，小鼠的活动次数显著增加(P＜0．05)，其脑组织匀浆中的Ach的含量为8．64±1．43pmol／g，较对照组明显增高(P＜0．05)，cck-8的含量较对照组略低。结论：多动症的发病与Ach和cck-8的变化有密切关系。%Objective: To detect the changes of acetylcholine(Ach) and cholecystokinin octapeptide(cck-8) in brain of hyperactive mice, in order to find the mechanism of ADHD. Methods: Ach and cck-8 level were determined by radioimmunity in hyperactive mice.Results: Mice were more hyperactive than those in control mice after IP scopolamine hydrobromide(3mg/kg) 30P?.05). The content of Ach in brain of hyperactive mice was 8.64±1.43 pmol/g,and it was markedly increased than those in control mice (P?.05), the content of cck-8 was decreased.Conclusion: The results indicated that changs of Ach and cck-8 related closely to mechanism of ADHD.