Objective To investigate the clinical significance of combination detection of AFP-L3, GPC3 and AFU in the diagnosis and treatment of primary hepatocellular carcinoma( PHC ). Methods The concentrations of AFP-L3,GPC3 ,AFU were detected by enzyme-linked immunosorbent assay( ELISA )in 100 patients with PHC ( PHC group ) ,50 patients with cirrhosis( cirrhosis group )and 50 healthy subjects( control group ) ,then the results were statistically analyzed. Results The positive rate of AFP-L3 in PHC group was significantly higher than that in cirrhosis group or control group( P <0.01 ),however,there was no significant difference between cirrhosis group and control group( P > 0. 05 ). The positive rate of GPC3 in PHC group was significantly higher than that in cirrhosis group or control group( P <0. 01 ),however, there was no significant difference between cirrhosis group and control group( P > 0. 05 ). The positive rate of AFU in PHC group was significantly higher than that in cirrhosis group or control group( P <0. 01 ),however, there was no significant difference between cirrhosis group and control group( P > 0. 05 ). The sensitivity, specificity and accuracy of combination detection of AFP-L3, GPC3, AFU were higher than those of any simple item detection. Conclusion The combination detection of AFP-L3 , GPC3 , AFU can enhance the sensitivity, specificity and accuracy in the diagnosis of PHC.%目的 探讨AFP-L3、GPC3、AFU三者联合检测模式对诊疗原发性肝癌(PHC)的效果.方法 选择肝癌患者100例(PHC组),肝硬化患者50例(肝硬化组),正常对照者50例(对照组),用酶联免疫法分别测出AFP-L3、GPC3和AFU 的浓度.然后分别按照检测项目进行统计分析.结果 (1)PHC组的AFP-L3阳性率明显高于肝硬化组和对照组(P<0.01);肝硬化组的阳性率与对照组差异无统计学意义(P>0.05).(2)PHC组的GPC3阳性率明显高于肝硬化组和对照组(P<0.01);肝硬化组的阳性率与对照组差异无统计学意义(P>0.05).(3)PHC组的AFU阳性率明显高于肝硬化组和对照组(P<0.01);肝硬化组阳性率与正常对照组差异有统计学意义(P<0.01).(4)AFP-L3、GPC3、AFU三者联合检测时,高于任何单项检测的敏感度、特异度和准确度.结论 AFP-L3、GPC3、AFU三者联合检测可以提高PHC诊断的敏感度、特异度和准确度.
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