Objective To observe the changes of cell proliferation activity and expressions of Galectin-3 , CyclinD1, p21 and p27 protein in Eca-109 cell line after transfected by Galectin-3 small interfering RNA ( siRNA ). Methods The Galectin-3 siRNA chain was constructed and transfected into Eca-109 cell line by lipofectamine 2000,then Real-time PCR,MTT,Flow cytometry and immuocytochemistry were respectively used to detect the transfection efficiency of siRNA, the cell proliferation activity, cell cycle distribution and the expression levels of Galectin-3, Cyclin D1 ,p21 and p27protein in Eca-109 cell line. Results The cell proliferation activity of Eca-109 cell line was significantly decreased, and cell distribution was mainly at G1 stage, and the expression levels of Galectin-3 and Cyclin Dl were decreased, however, the expression levels of p21 and p27 protein were increased. Conclusion The application of siRNA can effectively inhibit the expression of Galectin-3 and cell proliferation activity of Eca-109 cell line,and Galectin-3 is involved in the proliferation of Eca-109 cell line and is closely correlated with the expressions of Cyclin D1, p21, p27 protein, which may become the target gene for treating esophageal cancer.%目的 探讨siRNA干扰Galectin-3 mRNA后,食管癌Eca-109细胞株增殖活性的影响及Galectin-3、CyclinD1、p21、p27蛋白的表达.方法 将Galectin-3-siRNA转染食管癌Eca-109细胞株后,分别用Real-time PCR、MTT、流式细胞术和免疫细胞化学分别检测转染效率、细胞增殖活性、细胞周期分布及Galectin-3、CyclinD1、p21、p27蛋白的表达.结果 食管癌Eca-109细胞增殖活性明显减少,主要分布在G1期,Galectin-3和 CyclinD1蛋白表达下降,p21和p27蛋白的表达升高.结论 运用siRNA 可有效抑制Galectin-3蛋白的表达及细胞增殖活性,Galectin-3参与食管癌Eca-109细胞株的增殖与CyclinD1、p21、p27蛋白关系密切,Galectin-3可成为治疗食管癌的靶基因.
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