Objective To investigate the expression of cyclophilin B(CypB) in cancer tissues and serum of patients with gastric cancer, and to explore its clinical significance.Methods A total of 130 patients were divided into observation group(n=80) and control group(n=50). Paraffin sections were made from cancer tissues and paracancerous gastric mucosa tissues(over 5 cm away from edges of cancers) of all subjects and stained by immunohistochemistry to detect the expression levels of CypB in tumor tissues and paracancerous gastric mucosa tissues. Moreover the serum levels of CypB,CEA,IL-17 at 1d before treatment and at 1 month after treatment were detected by ELISA. In addition, the clinical pathological data of patients were also collected and the clinical staging was carried out according to TNM staging (7th version,2010 by UICC). Results The positive rates of CypB in cancer tissues were significantly higher than those in paracancerous gastric mucosa tissues (P <0.05). There were 11 cases of stageⅠ,21 cases of stage Ⅱ,42 cases of stage Ⅲ,6 cases of stage Ⅳ, respectively. The positive rates of CypB were increased in order of stageⅠto stage Ⅲ (P <0.05),but there were no significant differences in positive rates of CypB between stage Ⅲ group and stage Ⅳ group (P>0.05). The serum levels of CypB of patients before operation in observation group were significantly higher than those in control group (P<0.05). The serum levels of CypB were increased in order of stage I to stageⅣ(P<0.05). At one month after operation,the serum levels of CypB in observation group were decreased significantly, as compared with those in control group (P <0.05). Before operation,the serum levels of CypB in observation group were significantly higher than those in control group (P<0.05). After operation,the serum levels of CypB in observation group were significantly decreased, as compared with those before operation (P<0.05),however,there were no significant differences in serum levels of CEA and IL-17 between two groups (P>0.05).In addition the cancer tissues were closely correlated to the serum levels of CypB before operation(P<0.05), moreover,before operation the serum levels of CypB were closely correlated to those of CEA,IL-17(P<0.05).Conclusion The overexpression of CypB in serum and tumor tissues of patients with gastric cancer is significant in evaluating patient's prognosis.%目的 检测亲环素B(cyclophilin B,CypB)在胃癌肿瘤组织与血清中的表达情况,并对其临床意义进行探讨.方法 将收治的80例胃癌手术患者作为研究组,均有术后病理确诊,取肿瘤组织及距肿瘤边缘5 cm以上的胃黏膜组织制备石蜡切片,行免疫组化染色,检测肿瘤组织与癌旁组织中 CypB 的表达情况.酶联免疫吸附试验(ELISA)检测研究组患者术前1 d、术后1个月时血清中CypB、CEA、白介素17(IL-17)的含量.同时选取健康体检者50例作为对照组,取血清进行上述指标的检测.收集患者的临床病理资料,按UICC(2010年第7版)的TNM分期记录患者的肿瘤分期.结果 患者肿瘤组织中CypB表达的阳性率明显高于癌旁胃黏膜组织(P<0.05).患者中Ⅰ期11例、Ⅱ期21例、Ⅲ期42例、Ⅳ期6例.肿瘤组织中CypB表达的阳性率在Ⅰ~Ⅲ期中依次升高(均P<0.05),但Ⅳ期CypB表达的阳性率与Ⅲ期无明显差异(P>0.05).胃癌患者术前血清CypB的浓度均高于正常对照组(均P<0.05).分期Ⅰ~Ⅳ期患者血清CypB浓度依次升高,Ⅳ期者最高,Ⅰ期者最低(均P<0.05);术后1个月外周血CypB水平与对照组比较,差异有统计学意义(P<0.05).术后Ⅰ~Ⅳ期患者血清CypB浓度与相应分期术前相比水平明显下降(P<0.05).研究组术前血清中CEA、IL-17的水平与对照组比较差异有统计学意义(P<0.05);手术后胃癌患者血清中CEA、白介素的水平与术前相比均有明显变化(P<0.05),与对照组比较差异无统计学意义(P>0.05).胃癌患者肿瘤组织与手术前血清CypB水平有明显相关性(P<0.05).术前血清CypB水平与CEA、IL-17均存在相关关系(均P<0.05).结论 在胃癌患者血清及肿瘤组织CypB表达增高,检测胃癌患者CypB水平对病情评估有重要价值.
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