黄芪桂枝五物汤中总黄酮对小鼠免疫功能影响的体外研究

摘要

目的：探讨黄芪桂枝五物汤中总黄酮对小鼠免疫功能的影响，从而为临床应用提供参考。方法取小鼠脾淋巴细胞，加入不同浓度的总黄酮，采用水溶性四唑盐（ WST-1）法测定细胞的增殖活性；酶联免疫吸附试验（ELISA）测细胞因子白细胞介素-2（IL-2）和干扰素γ（IFN-γ）含量；流式细胞仪检测小鼠脾细胞中CD3＋、CD19＋细胞。结果各浓度总黄酮组淋巴细胞增殖活性、IL-2、IFN-γ及CD3＋细胞显著高于对照组（P＜0．05，P＜0．01））。结论总黄酮可增强免疫功能，但只能提高细胞免疫功能，对体液免疫功能无明显影响。%Objective To research the effect of total flavones extracted from Huangqi guizhi wuwu decoction on immune function mice on in vitro ,and to provide a reference for the clinical application .Methods Splenic lym-phocyte of mice were extracted ,and stimulated with different concentration flavones .Proliferations of the cells were de-tected with WST-1 assay;IL-2,IFN-γsecreted by the cells were examined with enzyme linked immune sorbent assay ( ELISA);Cell subtypes were checked with flow cytometry ( FCM ) .Results Compared with the control group,proliferation of the cells and concentration of IL -2,IFN-γand CD3 +in different concentration total flavones group increased significantly (P<0.05,P<0.01).Conclusion The total flavones might enhance cellular immune function but not humoral immune function .