干扰素Ⅱ型

摘要： Objective To study the effect of diosgenin(Dio) from Dioscorea nipponica on the proliferation in mouse spleen T lymphocytes and expressions of IL-2 and IFN-γmRNA,in order to investigate the immunity regulatory mechanism of Dio.Methods T lymphocytes stimulated by different concentrations of Dio and concanavalin A(ConA) were co-cultured.CCK-8 was used to detect the Dio effects on T lymphocyte proliferation.The RT-PCR method was adopted to detect the effect of Dio on expression of IL-2 and IFN-γmRNA.Results The Dio concentration in the range of 0.937 5-15.000 0 μg/mL had the inhibiting effect on T lymphocyte proliferation,Dio concentration in the range of 3.750 0-15.000 0μg/mL had the inhibiting effect on IFN-γ and IL-2 expression in T lymphocytes.With the Dio concentration increase,the inhibition effect was enhanced(P＜0.05).7.500 0 μg/mL was the best inhibition concentration.The inhibition effect was decreased when the concentration exceeding 7.500 0 μg/mL.Conclusion Dio has the inhibiting effect on T lymphocyte proliferation and expressions of IL-2 and IFN-γ mRNA.%目的 研究穿龙薯蓣皂苷元(Dio)对小鼠脾脏T淋巴细胞增殖及白细胞介素-2(IL-2)、干扰素-7(IFN-γ) mRNA表达的影响,探讨Dio的免疫调节机制.方法 以不同浓度Dio与刀豆蛋白A(ConA)刺激的T淋巴细胞共同培养,用CCK-8检测Dio对T淋巴细胞增殖的影响,反转录PCR法检测Dio对IL-2、IFN-γ mRNA表达的影响.结果 Dio浓度在0.937 5～15.000 0μg/mL范围,对T淋巴细胞增殖有抑制作用,在3.750 0～15.000 0 μg/mL范围,对T淋巴细胞IL-2、IFN-γ mRNA的表达有抑制作用,随Dio浓度增加抑制作用均增强(P＜0.05),7.500 0 μg/mL为最佳抑制浓度,超过此浓度抑制作用减弱.结论 Dio对T淋巴细胞的增殖及IL2、IFN-γ mRNA的表达均有抑制作用.

摘要： 目的 观察急性脑出血患者早期外周血干扰素-γ(IFN-γ)分泌水平与病情预后的关系.方法 选取2015年7月至2016年8月在该院急诊科住院的急性脑出血患者66例作为试验组,体检中心健康人群60例作为空白对照组,分离其外周血单个核淋巴细胞(PBMC)及血浆,ELISA检测辅助T细胞(Th) 1/Th2类细胞因子[IFN-γ、白细胞介素(IL)-2、11-4及IL-10];流式细胞术检测胞内细胞因子;NIHSS量表分析试验组发病第1天及第30天病情变化;分析细胞因子与第30天NIHSS量表评分预后的关系.结果 两组外周血中IFN-γ差异有统计学意义(P＜0.05),IL-2、IL-4、IL-10差异无统计学意义(P＞0.05).试验组早期外周血IFN-γ分泌水平与预后之间存在正向相关(P＜0.05).结论 早期外周血IFN-γ水平增高与急性脑出血的预后不良相关.%Objective To observe the relationship between the peripheral blood IFN-γ secretion level and prognosis in the patients with early stage of acute cerebral hemorrhage.Methods Sixty-six inpatients with acute intracerebral hemorhage in the emergency department of this hospital from July 2015 to August 2016 were selected as the experimental group,and 60 healthy people in the physical examination center served as the blank control group.The peripheral blood mononuclear cells(PBMCs) and plasma were separated.The helper T cells(Th)1/Th2 eytokines(IFN-γ,IL-2,IL-4 and IL-10) were detected by using ELISA.The flow cytometry was used to detect intracellular cytokines;the disease condition change from 1 d of onset to 30 d in the experiment group was analyzed by using NIHSS.The relationship between the cytokines and prognosis on 30 d evaluated by NIHSS score was analyzed.Results The peripheral blood IFN-γ level had statistically significant difference between the two groups(P＜0.05),while the levels of IL-2,IL-4 and IL-10 had no statistical difference(P＞0.05).The peripheral blood IFN-γ secretion level during early stage in the experimental group was positively correlated with the prognosis(P＜0.05).Conclusion The increase of peripheral blood IFN-γ during early stage is correlated with the poor prognosis in the patients with acute intracerebral hemorrhage.

摘要： 目的 探讨腹泻型感染后肠易激综合征(PI-IBS)患者外周血和肠黏膜细胞因子表达变化及其与临床症状积分的关系.方法 选择2013年1-12月海南省人民医院门诊及住院的腹泻型PI-IBS患者30例(观察组),同期健康体检者30例(对照组).分离培养两组对象的外周血单个核细胞(PBMC),ELISA检测外周血及细胞培养上清波中干扰素γ(IFN-γ)、白细胞介素(IL)-10水平;结肠镜取结肠黏膜组织,免疫组织化学检测结肠黏膜IFN-γ及IL-10蛋白表达.Spearman相关分析IFN-γ、IL-10水平变化与临床症状积分的相关性.结果 两组时象外周血中IL-10、IFN-γ水平比较,差异无统计学意义(P＞0.05);与对照组比较,观察组患者PBMC分离培养液中IFN-γ水平升高,IL-10水平降低,差异有统计学意义(P＜0.01).观察组患者肠道主要症状积分与PBMC培养上清液及结肠黏膜中IFN-γ表达水平呈正相关(r=0.45、0.94,P＜0.01),与IL-10表达水平呈负相关(r=-0.52、-0.79,P＜0.01).结论 IFN-γ/IL-10失衡可能参与腹泻型PI-IBS发病,并可作为疾病活动的观察指标.%Objective To investigate the expression change of cytokines in peripheral blood and intestinal mucosa in the patients with diarrhea post-infectious irritable bowel syndrome(PI-IBS) and its relation with clinical symptoms scores.Methods Thirty outpatients and inpatients with diarrhea PI-IBS(observation group) and contemporaneous 30 individuals undergoing physical examination(control group) in the Hainan Provincial People's Hospital from January to December 2013 were selected.The peripheral blood mononuclear cells(PBMC) were separated and cultured.Then the levels of IFN-y and IL-10 in peripheral blood and cell culture supernatant fluid were detected by ELISA.The colonic mucosal tissue was taken by coloscopy.Then colonic mucosal IFN-γ and IL-10 protein expression was detected by immunohistochemistry staining.Furthermore,the correlationship between the level change of IFN-γ and IL-10 with clinical symptom score was analyzed by using the Spearman correlation method.Results Peripheral blod IL-10 and IFN-γ levels had no statistical difference between the two groups(P＞0.05).Compared with the control group,in PBMC seperation and cuture,the IFN-γ level in the observation group was increased and IL-10 level was decreased,the difference was statistically signifieant(P＜0.01).The intestinal main symptom score in the observation group had the positive correlation with IFN-γ expression level of PBMC culture supernatant fluid and colonic mucosal IFN-γ expression level(r=0.45,0.94,P＜0.01),and had the negative correlation with IL-10 expression level(r=-0.52,-0.79,P＜0.01).Conclusion The unbalance of IFN-γ and IL-10 level could be involved in the pathogenesis of diarrhea PI-IBS,which can serve as the observation indicators of disease activity.

摘要： 目的 观察健脾疏肺食疗方对肺脾两虚证支气管哮喘(以下简称哮喘)大鼠肺泡灌洗液(BALF)中白细胞介素4(IL-4)和干扰素γ(IFN-γ)含量的影响.方法 将40只大鼠随机分为4组,分别为正常对照组、模型组、氨茶碱组、食疗组,每组各10只.除正常对照组外,模型组、氨茶碱组及食疗组大鼠以香烟喷雾、番泻叶浸液灌胃及卵清白蛋白磷酸缓冲液雾化吸入致肺脾两虚证哮喘模型.从造模开始第2d,正常对照组、模型组及氨茶碱组大鼠予0.9％氯化钠注射液2 mL灌胃,其中在造模第21 d氨茶碱组将0.9％氯化钠注射液更换为氨茶碱液2 mL(生药含量为4.06 mg)持续灌胃.食疗组在造模开始第2d予健脾疏肺食疗方药液2 mL(生药含量为2.87 g)持续灌胃.以上各组均持续灌胃至造模第30 d.比较4组大鼠实验第1d及灌胃结束时体质量的差值,检测比较BALF中IL-4和IFN-γ含量.结果 与正常对照组比较,模型组、氨茶碱组及食疗组大鼠体质量差值较小(P＜0.05),大鼠体质量增加幅度有所降低;与模型组比较,氨茶碱组及食疗组大鼠体质量差值增加(P＜0.05).与正常对照组比较,模型组、氨茶碱组及食疗组大鼠BALF中的IL-4含量均升高(P＜0.05),IFN-γ含量均降低(P＜0.05);与模型组比较,氨茶碱组及食疗组IL-4含量均降低(P＜0.05),IFN-γ含量均升高(P＜0.05).结论 健脾疏肺食疗方能借助调节细胞因子IFN-γ以及IL-4的含量,抑制或阻断哮喘的早期发病过程,抗气道变态反应性炎症及免疫调节作用可能是其防治哮喘的机制之一.

摘要： 目的 分析慢性荨麻疹与辅助性T细胞22(Th22)细胞免疫之间的相关性.方法 选取山东省泗水县人民医院检验科2014年8月至2017年4月收治的82例慢性荨麻疹患者作为观察组,82例体检健康者作为对照组.参照荨麻疹活动度评分(UAS)将慢性荨麻疹患者进一步分为轻度患者(总评分 ≤4分)与重度患者(总评分>4分).检测两组研究对象血清 γ-干扰素(IFN-γ)、白细胞介素(IL)-6、IL-22水平并进行比较,同时比较轻度患者与重度患者血清IFN-γ 、IL-6、IL-22水平.分析血清IFN-γ 、IL-6、IL-22水平与慢性荨麻疹的相关性.结果 观察组患者血清IFN-γ 、IL-6、IL-22水平[(2.42±0.49)、(2.30±0.62)、(116.24±22.55)pg/L]均显著高于对照组[(1.65±0.35)、(1.81±0.54)、(93.35±20.10)pg/L],差异均有统计学意义(P<0.05),重度患者血清IFN-γ 、IL-6、IL-22水平[(2.61±0.53)、(2.44±0.67)、(121.07±19.88)pg/L]均显著高于轻度患者[(2.22±0.47)、(1.96±0.59)、(111.03±15.89)pg/L],差异均有统计学意义(P<0.05).血清IFN-γ 、IL-6、IL-22水平均与慢性荨麻疹呈明显正相关(r依次为0.719、0.579、0.633,P<0.001).结论 慢性荨麻疹患者血清IFN-γ 、IL-6、IL-22水平均明显偏高,且病情越严重,血清IFN-γ 、IL-6、IL-22水平越高,与慢性荨麻疹的发病存在明显相关性.

摘要： 目的 探讨结核分枝杆菌相关γ-干扰素体外释放试验(TB-IGRA)在结核病诊断中的应用价值.方法 收集2015年4月至2016年4月在运城市中心医院进行TB-IGRA检测的门诊和住院患者共106例,其中肺结核、肺外结核患者共51例(结核病组),疑似结核及非结核病例55例(疑似结核及非结核组),观察两组患者结核阳性检出率,并对TB-IGRA与涂片镜检2种检测方法进行比较.结果 结核病组结核阳性检出率显著高于疑似结核及非结核组(χ2=56.3,P<0.01);TB-IGRA在肺结核和肺外结核病例中的阳性检出率高达93.3％(28/30)和95.2％(20/21),对结核病的阳性检出率[94.1％(48/51)]显著高于涂片镜检[27.5％(14/51)].结论 TB-IGRA在结核病诊断中具有较高的敏感性和特异性,是一种适合运城地区结核病诊断的重要辅助手段.

摘要： [Objective]To observe effects of total glucosides of paeony and tripterygium glycosides on serum IL-12, TGF-β and IFN-γ expression levels and immunologic function in patients with oral lichen planus (OLP).[Methods]The clinical data of 64 patients with OLP treated in our stomatology and dermatology divisions from March 2010 to March 2012 were retrospectively analyzed and divided into the control group and the treatment group according to the treatment method.Patients in the control group were given oral total glucosides of paeony capsules while those in the observation group were given oral tripterygium glycosides tablets.The clinical effect in the two groups after the treatment were compared.Enzyme linked immunosorbent assay was applied to detect IL-12, TGF-β and IFN-γ expressions in serum of two groups before and after the treatment, while flow cytometry was used to detect cellular immunity markers CD4+, CD8+, CD4+/CD8+ and other T lymphocyte subsets changes.[Results]The total effective rate was 84.37% (27/32) in the observation group, which was significantly higher than that in the control group (65.63%, 21/32);the difference was statistically significant (X2=7.682,P<0.05).After treatment, the expressions of IL-12 and IFN-γ in the serum of the observation group were significantly lower than those of the control group, while the expression of TGF-β was significantly higher than that of the control group (P<0.05).The immunity markers CD4+ and CD8+ in the observation group after the treatment were significantly lower than those in the control group, while CD4+/CD8+ was significantly higher than the control group (P<0.05).During the half-year follow-up, the recurrence rate was 9.38% (3/32) in the observation group, which was significantly lower than that in the control group (21.88%, 7/32);the difference was statistically significant (X2=6.552,P<0.05).The incidence of adverse reactions in the observation group was 25.00% (8/32), which was significantly higher than that in the control group (9.38%, 3/32) (X2=7.421,P<0.05).[Conclusion]The effect of total glucosides of paeony and tripterygium glycosides is significant in the treatment of oral lichen planus.They play a role by regulating cellular immunity, influencing expression and secretion of related cytokines.The efficacy of tripterygium glycosides is better than total glucosides of paeony.%[目的] 观察白芍总苷(TGP)与雷公藤多苷对口腔扁平苔藓(OLP)患者血清白细胞介素-12(IL-12)、转化生长因子β(TGF-β)、γ-干扰素(IFN-γ)表达水平及免疫功能的影响.[方法] 回顾性分析2010年3月至2012年3月本院口腔科与皮肤科收治的OLP患者64例,根据相关临床资料和OLP患者适应证分为对照组和观察组.对照组患者口服TGP胶囊,观察组患者口服雷公藤多苷片.比较两组患者治疗后的临床疗效及治疗前后血清中IL-12、TGF-β、IFN-γ表达水平、细胞免疫指标CD4+、CD8+和CD4+/CD8+等T淋巴细胞亚群的变化.[结果] 观察组患者总有效率为84.37%(27/32),明显高于对照组65.63(21/32),其差异具有统计学意义(X2=7.682,P<0.05).观察组患者治疗后血清中IL-12和IFN-γ表达水平明显低于对照组,而TGF-β表达水平明显高于对照组,其差异有统计学意义(P<0.05).治疗后观察组患者免疫指标CD4+和CD8+均明显低于对照组,而CD4+/CD8+免疫指标明显高于对照组,其差异均有统计学意义(P<0.05).随访半年发现,观察组复发率为9.38%(3/32),明显低于对照组21.88%(7/32),其差异有统计学意义(X2=6.552,P<0.05).观察组不良反应发生率为25.00%(8/32),明显高于对照组9.38%(3/32)(X2=7.421,P<0.05).[结论] TGP与雷公藤多苷治疗口腔扁平苔藓效果显著,两者均可通过调节机体细胞免疫和影响相关细胞因子的表达及分泌来发挥作用.二药相比,雷公藤多苷治疗效果更好,但毒副作用大,为后期两者联合用药提供可能,值得进一步临床研究.

摘要： 目的 探究人参白虎汤对1型糖尿病(T1DM)幼鼠血糖及干扰素γ(IFN-γ)、白细胞介素4(IL-4)的影响作用.方法 将50只幼鼠随机选取10只设为正常组,剩余40只以链脲佐菌素(STZ)腹腔注射1次诱导T1DM模型,造模成功后随机分为模型组、胰岛素组、人参白虎汤高剂量组及人参白虎汤低剂量组,每组各10只.胰岛素组予精蛋白生物合成人胰岛素注射液2 U/(kg·d)皮下注射,人参白虎汤高、低剂量组分别予人参白虎汤12、6 mL/(kg·d)灌胃,正常组及模型组予0.9％氯化钠注射液1 mL灌胃,均持续6周.观察5组幼鼠给药前后体质量变化,分别于给药前及给药结束后采集5组幼鼠尾静脉血测定空腹血糖,处死幼鼠取腹主动脉血采用酶联免疫吸附(ELISA)法检测IFN-γ和IL-4水平,并观察5组幼鼠胰腺的病理变化.结果 给药前,模型组、胰岛素组、人参白虎汤高剂量组及人参白虎汤低剂量组幼鼠体质量均较正常组明显下降(P＜0.05),空腹血糖升高(P＜0.05);给药后,胰岛素组、人参白虎汤高剂量组及人参白虎汤低剂量组幼鼠体质量均较本组给药前及模型组同期升高(P＜0.05),空腹血糖降低(P＜0.05),但与正常组同期比较差异仍有统计学意义(P＜0.05);与胰岛素组相比,人参白虎汤高剂量组体质量及空腹血糖无明显差异(P＞0.05),而人参白虎汤低剂量组体质量低,空腹血糖高(P＜0.05).与正常组相比,模型组、胰岛素组、人参白虎汤高剂量组及人参白虎汤低剂量组IFN-γ水平升高,IL-4水平降低(P＜0.05);与模型组相比,胰岛素组、人参白虎汤高剂量组及人参白虎汤低剂量组IFN-γ水平明显降低(P＜0.05),IL-4水平明显升高(P＜0.05),且人参白虎汤高剂量组与胰岛素组相比比较差异无统计学意义(P＞0.05).胰腺组织病理观察显示,胰岛素组、人参白虎汤高剂量组及人参白虎汤低剂量组胰岛β细胞损伤较模型组减轻,但仍未恢复正常.结论 人参白虎汤能明显降低T1DM幼鼠血糖、IFN-γ水平,提高IL-4水平,改善胰腺病理结构,进而发挥修复T1 DM幼鼠胰岛免疫损伤的作用.%Objective To observe the effects of Renshen-baihu decoction on plasma glucose,IFN-γ and interleukin-4 (IL-4) of immature rats with type 1 diabetes mellitus (T1DM).Methods 10 rats were randomly selected from 50 rats as the normal group,the remaining 40 rats were injected intraperitoneally with streptozotocin (STZ) to induce T1DM model.After successful modeling,the rats were randomly divided into model group,insulin group,high dose of Renshen-baihu decoction group and low dose of Renshen-baihu decoction group,and 10 rats ineach group.The insulin group was was injected subcutaneously with insulin by 2 U/ (kg · d).The high and low dose of Renshen-baihu decoction groups were respectively given Renshen-baihu decoction gavage by 12 mL / (kg · d) and 6 mL/(kg · d).The normal group and model group were given 1 mL0.9％ sodium chloride injection gavage.The treatment course was continuously for 6 weeks.The changes of body weight of immature rats before and after administration were observed in 5 groups.The tail vein blood was collected to detect fasting blood glucose before and after the administration in 5 groups.The abdominal aorta blood was collected to detect he levels of IFN-γ and IL-4 by enzyme-linked immunosorbent assay (ELISA) and the pathological changes of pancreas of immature rats were observed.Results The body weight before administration in model group,insulin group,high dose of Renshen-baihu decoction group and low dose of Renshen-baihu decoction group were significantly lower than those in normal group (P ＜ 0.05),and fasting blood glucose increased (P ＜ 0.05).The body weight after administration in the insulin group,high dose of Renshen-baihu decoction group and low dose of Renshen-baihu decoction group were higher than those before administration and those in the model group at the same period (P ＜ 0.05),and the blood glucose was decreased (P ＜ 0.05),and had statistical differences with the normal group at the same period (P ＜ 0.05).Compared with the insulin group,there was no significant difference on the body weight and fasting blood glucose in the high dose of Renshen-baihu decoction group (P ＞ 0.05),while the the body weight in the low-dose of Renshen-baihu decoction group was lower and the fasting blood glucose was still higher (P ＜ 0.05).Compared with the normal group,the IFN-γγ increased in model group,insulin group,high dose of Renshen-baihu decoction group and low dose of Renshen-baihu decoction group,and IL-4 reduced (P ＜ 0.05).Compared with the model group,the IFN-γ in insulin group,high dose of Renshen-baihu decoction group and low dose of Renshen-baihu decoction group were significantly decreased (P ＜ 0.05),IL-4 was significantly increased (P ＜ 0.05),and there was no significant difference between high dose of Renshen-baihu decoction group and insulin group (P ＞ 0.05).The observation of pancreatic tissue histopathology showed that the injury of islet β cell in the insulin group,high dose of Renshen-baihu decoction group and low dose of Renshen-baihu decoction group were less than that in model group,but still not returned to normal.Conclusion Renshen-baihu decoction can significantly reduce the blood glucose,IFN-γ,and increase IL-4 in immature rats with T1 DM,improve the pathological structure of the pancreas,and thus can play a role in the repair of islet immune injury in immature rats.

摘要： 用于确定患者对包括I型干扰素的施用的疗法的响应性的方法，以便做出用I型干扰素治疗患者的决定，以及IFNAR2中的SNP rs9984273(C/T)作为用于确定患者对所述疗法的响应性的标记物的用途。

摘要： 本发明涉及一种增强I型IFN对靶细胞群中的细胞的抗生长作用的方法，包括提高靶细胞群内修饰的细胞的表面上功能性IFNAR2c受体的数目，然后将修饰的细胞暴露于治疗有效量的I型IFN或暴露于内源性产生的IFN。

摘要： 本发明公开了一种由犬白细胞介素2、犬干扰素γ和犬干扰素α组成的融合蛋白及其制备方法，所述融合蛋白由犬白细胞介素2、犬干扰素γ和犬干扰素α经柔性linker连接而形成，融合蛋白与冻干保护剂混配后经冷冻干燥即可得到重组犬长效干扰素。所述重组犬长效干扰素可显著提高犬干扰素的半衰期，较普通犬干扰素的半衰期提高15倍以上，并具有广谱抗病毒作用并能提高犬自身的免疫应答。

摘要： 本发明公开了一种由猪白细胞介素2、猪干扰素γ和猪干扰素α组成的融合蛋白及其制备方法，所述融合蛋白由猪白细胞介素2、猪干扰素γ和猪干扰素α经柔性linker连接而形成，融合蛋白与冻干保护剂混配后经冷冻干燥即可得到重组猪长效干扰素。所述重组猪长效干扰素可显著提高猪干扰素的半衰期，较普通猪干扰素的半衰期提高19倍以上，并具有广谱抗病毒作用并能提高猪自身的免疫应答。

摘要： 本发明公开了一种由羊白细胞介素2、羊干扰素γ和羊干扰素τ组成的融合蛋白及其制备方法，所述融合蛋白由羊白细胞介素2、羊干扰素γ和羊干扰素τ经柔性linker连接而形成，融合蛋白与冻干保护剂混配后经冷冻干燥即可得到重组羊长效干扰素。所述重组羊长效干扰素可显著提高羊干扰素的半衰期，较普通羊干扰素的半衰期提高15倍以上，并具有广谱抗病毒作用并能提高羊自身的免疫应答。

摘要： 本发明公开了一种由鸡白细胞介素2、鸡干扰素γ和鸡干扰素α组成的融合蛋白及其制备方法，所述融合蛋白由鸡白细胞介素2、鸡干扰素γ和鸡干扰素α经柔性linker连接而形成，融合蛋白与冻干保护剂混配后经冷冻干燥即可得到重组鸡长效干扰素。所述重组鸡长效干扰素可显著提高鸡干扰素的半衰期，较普通鸡干扰素的半衰期提高18倍以上，并具有广谱抗病毒作用并能提高鸡自身的免疫应答。

摘要： 本发明公开了一种由牛白细胞介素2、牛干扰素γ和牛干扰素α组成的融合蛋白及其制备方法，所述融合蛋白由牛白细胞介素2、牛干扰素γ和牛干扰素α经柔性linker连接而形成，融合蛋白与冻干保护剂混配后经冷冻干燥即可得到重组牛长效干扰素。所述重组牛长效干扰素可显著提高牛干扰素的半衰期，较普通牛干扰素的半衰期提高16倍以上，并具有广谱抗病毒作用并能提高牛自身的免疫应答。

摘要： 本发明公开了一种由羊白细胞介素2、羊干扰素γ和羊干扰素τ组成的融合蛋白及其制备方法，所述融合蛋白由羊白细胞介素2、羊干扰素γ和羊干扰素τ经柔性linker连接而形成，融合蛋白与冻干保护剂混配后经冷冻干燥即可得到重组羊长效干扰素。所述重组羊长效干扰素可显著提高羊干扰素的半衰期，较普通羊干扰素的半衰期提高15倍以上，并具有广谱抗病毒作用并能提高羊自身的免疫应答。

摘要： 本发明公开了一种由鸡白细胞介素2、鸡干扰素γ和鸡干扰素α组成的融合蛋白及其制备方法，所述融合蛋白由鸡白细胞介素2、鸡干扰素γ和鸡干扰素α经柔性linker连接而形成，融合蛋白与冻干保护剂混配后经冷冻干燥即可得到重组鸡长效干扰素。所述重组鸡长效干扰素可显著提高鸡干扰素的半衰期，较普通鸡干扰素的半衰期提高18倍以上，并具有广谱抗病毒作用并能提高鸡自身的免疫应答。

摘要： 本发明公开了一种由牛白细胞介素2、牛干扰素γ和牛干扰素α组成的融合蛋白及其制备方法，所述融合蛋白由牛白细胞介素2、牛干扰素γ和牛干扰素α经柔性linker连接而形成，融合蛋白与冻干保护剂混配后经冷冻干燥即可得到重组牛长效干扰素。所述重组牛长效干扰素可显著提高牛干扰素的半衰期，较普通牛干扰素的半衰期提高16倍以上，并具有广谱抗病毒作用并能提高牛自身的免疫应答。