分别以乙酸钠(1.8∶1)、柠檬酸钠(3.5∶1)、葡萄糖(3∶1、6∶1、7∶1)、甲醇(3.2∶1)为唯一碳源,研究不同碳源及碳氮比对反硝化细菌活性的影响.结果表明,不同碳源及碳氮比情况下均能实现对硝酸盐的去除,其中,以乙酸钠为碳源时去除效率最高,10 h 去除率可达78.8%；以甲醇为碳源时去除效率最低,24 h去除率为71.9%.以柠檬酸钠和甲醇作为唯一碳源时,观察到亚硝酸盐的积累,峰值分别为16.9 mg ・ L -1、15.3 mg ・ L -1；以葡萄糖、乙酸钠作为唯一碳源时,亚硝酸盐氮含量较低.用单一底物 Monod 方程模拟不同碳源条件下 NO -3- N 去除情况,发现模拟值与实验值吻合良好.%The sodium acetate (1 .8 1) ,sodium citrate (3 .5 ∶ 1) ,glucose (3 ∶ 1/6 ∶ 1/7 ∶ 1) ,metha-nol (3 .2 ∶ 1) were chosen as sole carbon source respectively ,and the influences of different kinds of carbon source and carbon nitrogen ratio on denitrifying bacteria activities were investigated .The re-sults showed that system can achieved nitrate nitrogen removal under all conditions .Among them , when sodium acetate was chosen as sole carbon source ,the highest removal efficacy was achieved by 78 .8% .however ,when methanol was chosen as sole carbon source ,the lowest efficacy was achieved by 71 .9% .Nitrite nitrogen accumulation was observed when sodium citrate and methanol were used as the sole carbon source ,and peak concentration were 16 .9 mg ・ L - 1 and 15 .3 mg ・ L - 1 respectively . When glucose and sodium acetate were used as sole carbon source respectively ,nitrite nitrogen accu-mulation didn’t exist Monod equation was used to simulate the dynamics of NO 3 — N degradation un-der different carbon source conditions ,and the simulated values agreed well with the experimental val-ues .
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