护士外周血甲状腺激素、肾上腺皮质激素、HSP70及 PAI-1水平变化及意义

摘要

目的：检测护士外周血甲状腺激素、肾上腺皮质激素、HSP70及PAI-1含量，探讨其水平变化与亚健康状态的相关性。方法选取亚健康高危职业护士200人及性别、年龄配对非护士职业健康人群200人（对照组），采用ELISA法检测外周血中三碘甲状腺原氨酸（T3）、四碘甲状腺原氨酸（T4）、促甲状腺激素（TSH）、5-羟色胺（5-HT）、外周血热休克蛋白70（HSP70）及纤溶酶原激活物抑制物1（PAI-1）水平。结果与对照组相比，护士组外周血T3、TSH、5-HT含量下降，HSP70含量升高，差异均有统计学意义（P＜0．001）；T4、PAI-1含量虽有变化，但不存在统计学意义（P＞0．05）。护士亚健康状态与其外周血T3（r＝－0．778， P＝0．000）、TSH（r＝－0．745，P＝0．000）、5－HT （r＝－0．794，P＝0．000）存在显著负相关、与HSP70（r＝0．852，P＝0．000）存在显著正相关。结论亚健康状态可影响内分泌功能及应急蛋白表达，采用现代生物技术可对机体的“亚健康”状态进行定量评估。%ObjectiveTo examine the possible correlation of change in the levels of thyroid hormone , adrenal cortical hormone, HSP70 and PAI-1 in the peripheral blood of working nurses to their sub-health state.Method 200 working nurses were chosen as the experimental group while 200 non-nurse healthy individuals of age and gender similar to the experimental group were chosen as the control group .The respective levels of three triiodothyronine (T3), four triiodothyronine (T4), thyroid stimulating hormone (TSH), 5-(5-HT), and heat shock protein 70 (HSP70) and plasminogen activator inhibitor 1 (PAI-1) in the peripheral blood of the subjects of study were then detected with enzyme-linked immuno sorbent assay (ELISA).Findings As compared with those of the control group , the levels of T3, TSH, and 5-HT of the experimental group significantly decreased while the level of HSP 70 of the experimental group significantly increased .The difference was statistically significant (P0.05).There was a significant negative correlation between the sub-health status of nurses and the level of T3 (r=-0.778, P=0.000), TSH (r=-0.745, P=0.000), 5 -HT (r=-0.794, P=0.000).There was a significant positive correlation between the sub-health status of nurses and the level of HSP70 (r=0.852, P=0.000). Conclusion The sub-health status may affect the function of endocrine and the expression of stree protein .Modern biotechnology can be used for a quantitative assessment of the sub-health state of the body .