雷帕霉素抑制人肝癌细胞HepG-2侵袭能力的实验研究

摘要

To investigate the effect of cell invasion ability induced by rapamycin on human hepatocellular carcinoma HepG-2 ceils.Methods HepG-2 ceils in vitro were given different concentrations of rapamycin, and the invasion ability of HepG-2 ceils was observed by Matrigel. Results Rapamycin could inhibit the invasion ability of HepG-2 cells,and and the inhibit presented dose-dependent relationship. There was significantly different between rapamycin given groups and control group. Conclusion Rapamycin significantly inhibits the invasionability of HepG-2 cells.%目的 观察雷帕霉素(Rapamycin)体外对肝癌HepG-2细胞侵袭能力的影响.方法 用不同浓度的雷帕霉素作用于体外培养的HepG-2细胞,应用Matrigel胶模拟基质检测细胞侵袭能力.结果 雷帕霉素能显著降低肿瘤细胞的侵袭能力,随剂量增高降低越明显.各药物处理组与阴性对照组相比差异具有统计学意义(P<0.05).结论 雷帕霉素能有效地降低肝癌细胞HepG-2的侵袭能力.