过敏性疾病血清总IgE和过敏原特异性IgE检测的临床意义

摘要

Objective To investigate the clinical significance of the serum levels of allergen-specific IgE (sIgE) and total IgE (TIgE) in patients with allergic disease. Methods The serum levels of TIgE, Phadiatop and fx5E sIgE in 660 patients with allergic disease were detected by using fluoroenzyme-immunometrieassay and UniCAP100. Results The levels of TIgE in the patients of 17-84 years old was significantly higher than those in the patients of 0~ 3 and 4-16 years old (P<0.05). The total positive rate of TIgE was 93.0% (614/660), and the levels of TIgE were higher than 100 kU/L in 465 patients. The total positive rate of sIgE was 65.76% (434/660). 102 patients were positive in both Phadiatop and fx5E sIgE, in which 92 had TIgE values higher than 100 kU/L. The positive rate of fx5E was markedly higher in patients of 0~3 years old than those of 0~3 and 4-16 years old (P<0.05). The positive rates of Phadiatop were higher in patients of 4-16 years old and 17-84 years old (P?0.05). The positive rate of IV-VI rank of Phadiatop was higher than that of fx5E (P<0.05). Conclusion Detection of allergen by fluoroenzyme-immvinome-trieassay can not only indicate allergen constitution of patients, but also find relevant allergen, and provide assistance for the diagnosis and treatment of allergic disease.%目的 探讨血清总IgE(TIgE)和过敏原特异性IgE(sIgE)检测在过敏性疾病中的意义.方法 采用全自动过敏原体外检测系统UniCAP100检测660例过敏性疾病患者血清TIgE,吸入性过敏原过筛试验(Phadiatop)及食物过敏原过筛试验(fx5E)测定sIgE水平.结果 17～84岁组TIgE水平明显高于0～3岁组与4～16岁组(P＜0.05),660例过敏性疾病患者TIgE总阳性率为93.0％ (614/660),其中465例(75.73％)TIgE＞ 100 kU/L.sIgE总阳性率为65.76％ (434/660),吸入与食物过敏原均阳性者102例,其中92例TIgE＞ 100 kU/L.0～3岁组食物过敏原阳性率较另外两组显著增高(P＜0.01),4～16岁组和17～84岁组吸入性过敏原阳性率增高(P＜0.05).吸入性过敏原Ⅳ～Ⅵ级阳性率明显高于食物性(P＜0.005).结论 采用荧光酶联免疫法进行过敏原体外检测,不仅可以预示过敏体质,还可以查找过敏原因,为过敏性疾病的诊断和治疗提供帮助.