肠源性高草酸尿大鼠模型的建立

摘要

Objective To establish enterogenous hyperoxaluria model in rats. Methods Thirty randomly selected female Sprague-Daw-ley rats were fed in individual metabolic cage for 7 days. 24 h urine oxalate excretions were examined by ion chromotagraphy before and after the spinach juice lavage, and the difference was investigated. Another 20 female Sprague-Daw-ley rats were randomly divided into group A and group B. Group B was lavaged with spinach juice and group A was lavaged with equivalent pure water. 24 h urine oxalate excretions of group A and group B were compared. Results The 24 h urine oxalate excretion was (0.53±0.18) mg before lavaging spinach juice. On first day, third day and seventh day after lavaging spinach juice, the 24 h urine oxalate excretions were (1.93 ± 0.36) mg, (1.85 ± 0.38) mg, and (2.03 ± 0.39) mg,respectively. The 24 h urine oxalate excretion of group A and group B were (0.44±0.12) mg and (1.68±0.38) mg, respectively. Conclusion The 24 h urine oxalate excretion of SD rats was mark-edly increased 3 to 4 times after lavaged with spinach juice. Daily lavage with quantitatively and constant concentra-tion spinach juice can maintain long-term high oxalic acid urine state in rats. This method of establishing enterogenous hyperoxaluria model in rats is simple and feasible, which can be widely used.%目的：建立肠源性高草酸尿的大鼠模型。方法随机选取30只雌性SD大鼠置于大鼠代谢笼中适应性喂养7 d之后收集24 h尿，记录尿量，采用离子色谱仪测定其草酸浓度，计算正常大鼠在该条件下24 h尿草酸排泄量。菠菜汁灌胃处理大鼠，再测定灌胃后24 h尿草酸排泄量。对比灌胃菠菜汁前后，大鼠24 h尿草酸排泄量的差别；分组对照实验，随机选取20只雌性SD大鼠，随机分成A组和B组，B组灌胃菠菜汁，A组灌胃等量纯净水，其他条件相同，比较两组24 h尿草酸排泄量。结果反复测定，正常大鼠灌胃菠菜汁前24 h尿草酸排泄量为(0.53±0.18) mg，灌胃菠菜汁后第1天为(1.93±0.36) mg，第3天为(1.85±0.38) mg，第7天为(2.03±0.39) mg；A组(0.44±0.12) mg，B组(1.68±0.38) mg。结论大鼠在灌胃菠菜汁之后其尿液24 h草酸排泄量明显增加，大概增加3~4倍，说明每天对大鼠进行定量定浓度的菠菜汁灌胃，可以使其保持长期的高草酸尿状态，该方法建立的肠源性高草酸尿大鼠模型简便可行，可广泛用于其他方面的研究。