廉江市育龄期妇女AsAb、EmAb和AcAb抗体阳性率调查

摘要

目的：调查廉江市育龄期妇女抗精子抗体(AsAb)、抗子宫内膜抗体（EmAb）和抗心磷脂抗体（AcAb）阳性率，并比较两种检测方法的可靠性。方法分别以斑点免疫渗滤试验法（DIGFA）与酶联免疫吸附法（ELISA）检测2013年2月至2015年2月于我院行婚前检查的500例廉江市育龄期未生育妇女的AsAb、EmAb和AcAb情况，并比较两种检测方法的敏感度、特异度、符合度和重复率。结果廉江市育龄期妇女AsAb、EmAb、AcAb的ELISA法检测阳性率分别为2.0%、3.8%、1.6%，而DIGFA法检测阳性率分别为2.4%、4.6%、1.8%。DIGFA法检测3种抗体相对于ELISA法的敏感度为87.5%~90.0%，特异度为98.8%~99.6%，符合度为98.4%~99.4%，差异均无统计学意义(P>0.05)；DIGFA法的日间重复性为100.0%，明显高于ELISA法的94.0%，差异具有统计学意义(P<0.05)。结论廉江市育龄期妇女的3种抗体阳性率较低，DIGFA法检测结果与ELISA检测结果相当，但DIGFA法的稳定性要高于ELISA法，且其具有简便、快捷的特点，其在免疫性不孕的流行病学调查中具有重要的临床推广价值。%Objective To investigate positive rate of anti-sperm antibody (AsAb), anti-uterine endometrial an-tibody (EMAb) and anti-cardiolipin antibody (AcAb) in women of childbearing age Lianjiang City, and to compare the reliability of dot immunogold filtration test method (DIGFA) and enzyme-linked immunosorbent adsorption method (ELISA). Methods A total of 500 cases of women of childbearing age with AcAb, EmAb and AsAb level in Lianjiang city in our hospital from February 2013 to February 2015 were detected by DIGFA and ELISA. The sensitivity, specifici-ty, in line with the degree and repetition rate of the detection method were compared. Results The positive rates of As-Ab, EmAb, ELISA of women of childbearing age in Lianjiang city detected by ELISA were 2.0%, 3.8%, 1.6%, and the positive rates detected by DIGFA were 2.4%, 4.6%, 1.8%. The sensitivity of the DIGFA method for detecting 3 kinds of antibodies with respect to the ELISA method was 87.5%~90.0%, the specificity was 87.5%~90.0%, and the coincidence degree was 98.4%~99.4%, with no statistically significant difference (P>0.05). The day repeatability (100.0%) of DIG-FA method was significantly higher than that of ELISA (94.0%), and the difference was statistically significant (P<0.05). Conclusion The positive rate of 3 kinds of antibodies in women of childbearing age in Lianjiang city was low. The re-sults of DIGFA method and ELISA method are quite comparative, but the stability of DIGFA method is higher than that of ELISA method and its operation is simple and quick, which has an important value in the epidemiological investiga-tion of immune infertility.