目的 探讨Reprimo基因3'非翻译区824位点G>C单核苷酸多态性改变与肺癌发生的关系.方法 基因分型用TaKaRa的MightyAmp DNA聚合酶法,分别检测36例肺癌患者(癌症组)及其癌旁正常组织(对照组)的Reprimo基因3'非翻译区824位点G>C分布,运用χ2检验和Armitage's趋势检验等分析其与肺癌发生的相关性.结果 两组标本经Hardy-Weinberg平衡检测,对照组在Reprimo基因3'非翻译区824位点G>C基因型观测(理论)频数为34、1、1 (33.06、2.88、0.06),差异有统计学意义(P=0.042),癌症组的基因型观测(理论)频数为33、1、2 (33.17、4.56、0.17),差异有统计学意义(P=0.003);Reprimo基因3'非翻译区824位点G>C单核苷酸多态性与肺癌易感性的Armitage's趋势检验差异无统计学意义(χ2=0.31,P=0.581).结论 对照组接近符合Hardy-Weinberg遗传平衡状态,而肺癌组不符合该规律;Reprimo基因3'非翻译区824位点G>C单核苷酸位点多态性可能与肺癌发生不具有相关性.%Objective To investigate the relationship between 824 G>C polymorphism in the 3'-untranslated region of Reprimo gene and lung cancer. Methods Genotyping was performed by MightyAmp DNA Polymerase (Ta-KaRa) with samples of cancerous tissues (cancer group) and pericarcinous tissues (control group) from 36 lung cancer patients to detect the 824 G>C polymorphism in the 3'-untranslated region of Reprimo gene.χ2 test and Armitage's trend test were applied to analyze the correlation between 824 G>C polymorphism and the risk of lung cancer. Results Ac-cording to Hardy-Weinberg equilibrium detection, 824 G>C genotype observation (theory) frequency was 34, 1, 1 (33.06, 2.88, 0.06) in the control group (with statistically significant difference, P=0.042) and 33, 1, 2 (31.17, 4.56, 0.17) in the cancer group (with statistically significant difference, P=0.003). Armitage's trend test revealed no significant corre-lation between 824 loci G>C single nucleotide polymorphisms and susceptibility to lung cancer (χ2=0.31, P=0.581). Conclusion The control group was nearly accord with Hardy-Weinberg genetic equilibrium state, but lung cancer group was not. No association was found between 824 G>C polymorphism in the 3'-untranslated region of Reprimo gene and lung cancer.
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