Hi-efficient mammalian gene expression vectors containing exogenous promoter and the entire 5 'untranslated region upstream of the intrinsic gene start codon as a transcriptional regulatory site

摘要

The present invention relates to the highly efficient eukaryotic expression vectors which have regulatory sequences derived from human cytomegalovirus immediate early gene or human EF1 alpha gene, thereof transcriptional regulatory regions contain exogenous promoter and entire 5' untranslated region correspond to the condition of inherent gene expression. Therefore, the eukaryotic expression vectors provided in accordance with the present invention can drive high levels of gene expression in human and animal cells and have utilities as gene therapy agent.