子宫肌瘤组织信号通路分子及MMPs、IL-6、IL-10的表达及临床意义

摘要

目的 检测子宫肌瘤患者信号通路分子及MMPs、IL-6、IL-10及T细胞亚群相关指标并探讨其临床意义.方法 选取海南医学院附属医院妇科2013年1月至2016年10月收治的患子宫肌瘤而行子宫全切除术或子宫次全切除术患者68例作为观察组,选择同期60例因宫颈CINⅢ级行子宫切除术的患者作对照组,采用Western blot检测信号通路分子的含量,免疫组化法测定MMP-2、MMP-7、MMP-9、IL-6、IL-10的含量,S-P法对T细胞亚群相关指标进行检测分析.结果 观察组患者抗氧化信号通路分子ARE、Nrf2含量分别为(79.31±15.24)、(83.53±16.42),明显低于对照组的(227.35±27.17)、(173.41±20.15),增殖信号通路分子Ras、JNK、ERK含量分别为(87.36±13.75)、(92.16±15.31)、(95.47±15.03),高于对照组的(23.69±3.61)、(35.16±6.37)、(34.73±5.81),差异均有统计学意义(P<0.05).观察组血清中MMP-2、MMP-7、MMP-9含量分别为(35.24±3.78)、(25.74±4.63)、(38.56±4.39),均低于对照组的(84.57±17.39)、(94.75±16.57)、(85.73±17.41),观察组IL-6、IL-10水平分别为(28.96±0.68)、(16.37±6.42),低于对照组的(39.18±1.56)、(13.25±2.46),差异均具有统计学意义(P<0.05).观察组外周血CD4+、CD8+及CD4+/CD8+细胞水平分别为(39.63±9.64)％、(29.24±7.62)％、(1.32±0.18),均显著低于对照组(79.82±8.75)％、(38.72±6.21)％、(1.96±0.34),差异均有统计学意义(P<0.05).结论 MMPs、IL-6、IL-10及T细胞亚群相关指标可早期预测子宫肌瘤的发生.%Objective To investigate the indexes of signal pathway molecules and MMPs, IL-6, IL-10 and T-cell subgroups in patients with uterine fibroids and to explore their clinical significance. Methods Sixty-eight pa-tients who underwent total hysterectomy or subtotal hysterectomy for uterine fibroids Department of Obstetrics and Gy-necology of our hospital from January 2013 to October 2016 were selected as the observation group, and 60 patients who underwent hysterectomy for cervical intraepithelial neoplasia (CIN)Ⅲat the same time were enrolled in the con-trol group. Western blot was used to detect the contents of signaling pathway molecules, and the contents of MMP-2, MMP-7, MMP-9, IL-6, IL-10 were evaluated by immunohistochemistry and S-P method was used to determin the T-cell subgroups indexes. Results The contents of antioxidant signaling pathway molecules ARE and Nrf2 in the obser-vation group were (79.31±15.24) and (83.53±16.42), significantly lower than (227.35±27.17) and (173.41±20.15) in the control group, respectively (P<0.05). The contents of Ras, JNK and ERK in proliferation signaling pathway were (87.36± 13.75), (92.16±15.31) and (95.47±15.03), respectively, significantly higher than (23.69±3.61), (35.16±6.37) and (34.73± 5.81) in the control group (P<0.05). Serum levels of MMP-2, MMP-7 and MMP-9 were (35.24 ± 3.78), (25.74 ± 4.63) and (38.56 ± 4.39), respectively, significantly lower than (84.57 ± 17.39), (94.75 ± 16.57) and (85.73 ± 17.41) in the control group (P<0.05). The levels of IL-6, IL-10 in the observation group were (28.96 ± 0.68) and (16.37 ± 6.42), significantly lower than (39.18 ± 1.56) and (13.25 ± 2.46) in the control group, respectively (P<0.05). The data of CD4+, CD8+ cell counts and CD4/CD8 ratio in the peripheral blood of the observation group were (39.63 ± 9.64)％, (29.24 ± 7.62)％ and (1.32±0.18), significantly lower than (79.82±8.75)％, (38.72±6.21)％and (1.96±0.34) in the control group, respectively (P<0.05). Conclusion MMPs, IL-6, IL-10 and T-cell subgroups can predict the occurrence of uterine fibroids.