目的：研究益气温阳活血方对慢性心力衰竭大鼠心室重塑相关因子的影响。方法通过异丙肾上腺素（ISO）诱导慢性心力衰竭动物模型。选择健康Wistar大鼠40只，随机分为正常组、模型组、卡维地洛组（西药组）、益气温阳活血方组（中药组）；心脏超声检测心脏结构及功能，以酶联免疫吸附法检测去甲肾上腺素（N E ）、血管紧张素Ⅱ（AngⅡ）、内皮素‐1（ET‐1）、一氧化氮（NO）、金属基质蛋白酶‐2（MMP‐2）、金属基质蛋白酶‐9（MMP‐9）、金属基质蛋白酶抑制物‐1（TIMP‐1）、金属基质蛋白酶抑制物‐2（TIMP‐2）含量。结果模型大鼠左室后壁厚度（LVPW）、室间隔厚度（IVS）明显升高，射血分数（EF％值）则明显降低；NE、AngⅡ、ET‐1、MMP‐2、MMP‐9含量明显升高，而NO、TIMP‐1、TIMP‐2含量则明显降低，与正常组比较差异有统计学意义（P＜0．05）；益气温阳活血方可降低慢性心力衰竭大鼠NE、AngⅡ、ET‐1、MMP‐2、MMP‐9含量及升高NO、TIMP‐1、TIMP‐2含量，提高EF％值、降低IVS及LVPW厚度，与模型组比较差异有统计学意义（P＜0．05），而与正常组及卡维地洛组比较差异无统计学意义（P＞0．05）。结论益气温阳活血方可抑制心力衰竭大鼠相关心室重塑因子的水平，防止心室重塑。%Objective To study effects of Yiqiwenyanghuoxue decoction on myocardial remodeling associated fac‐tors of rat with chronic heart failure .Method The model rats with chronic heart failure were injected with ISO .40 healthy Wistarrats were randomly separated into 4 groups:normal group ,ISO‐injected group ,ISO‐injected with Carvedilol group and ISO‐injected with Yiqiwnyanghoxue group ,then cardiac structure and function were ?tested by echocardiography ,AngⅡ ,ET‐1 ,NO ,MMP‐2 ,MMP‐9 ,TIMP‐1 and TIMP‐2 were measured by ELISA .Re‐sult Compared with the normal group ,those were significantly higher at LVPW ,IVS NE ,NE ,Ang Ⅱ ,ET‐1 , MMP‐2 and MMP‐9 in ISO‐injected group ,but those were significantly lower at EF% ,NO ,TIMP‐1 and TIMP‐2 (all P<0 .05) ,and these change can be attenuated by decoction of yiqiwenyanghuoxue ,compared with the normal group and ISO‐injected with Carvediol group ,there were no significant differences in ISO‐injected with Yiqiwn‐yanghoxue group (all P> 0 .05) .Conclusion Yiqiwenyanghuoxue decoction may reverse the myocardial remodeling through regulating myocardial remodeling associated factors .
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