白术白绢病病原菌的分子鉴定

摘要

为了从分子水平上鉴定疑似引起白术白绢病的病原菌,有效控制白术白绢病的发生和危害,利用PCR方法并结合序列分析,对贵州省遵义县松林镇白术基地采集的疑似白绢病标样分离物进行PCR检测及序列分析.结果表明:疑似白绢病标样分离物扩增得到约700 bp的条带;序列分析结果表明,与已报道的Sclerotium rol f sii同源性达95％以上.因此,该病害确定为白绢病,病原菌为Sclerotium rol f sii.%The separator of the suspected southern blight sample collected from the A. Macrocephala base in Songlin Town, Zunyi County was identified by PCR and sequence analysis to determine the pathogen causing southern blight at the molecular level for effectively controlling occurrence and damage of southern blight in A. Macrocephala. The results showed that the band with about 700 bp was amplified from the separator of the suspected southern blight sample. The sequence analysis showed that the homology between the separator and the reported Sclerotium rolfsii reached above 95%.