甘薯再生体系的建立及试管结薯初探

摘要

为进一步提高甘薯脱毒快繁与试管薯的生产技术,以豫薯4号种薯催出芽苗的茎段与茎尖为外植体建立甘薯的再生体系,并对试管结薯进行了初步探索.结果表明:愈伤组织诱导最佳培养基为MS+TDZ 0.2 mg/L+NAA 0.1 mg/L+蔗糖30 g/L+琼脂5.8 g/L (pH 5.8);最佳愈伤组织分化培养基为MS+NAA 1.0 mg/L+蔗糖30 g/L+琼脂5.8 g/L(pH 5.8);不同品种与不同外植体愈伤诱导的比较中,出愈率与生长状况总体上豫薯4号＞豫薯7号＞遗306＞农大22,叶柄＞叶片＞茎尖＞茎段;试管结薯的试验中有根的膨大现象,但还未得到试管薯.%The regeneration system was established from stem segments and tips of Yushu 4, a sweet potato variety, to further improve the technique of detoxification, rapid propagation and microtuber production in sweet potato. The results showed that the optimum medium for callus induction and callus differentiation was MS+TDZ 0.2 mg/L+ NAA 0.1 mg/L+sucrose 30 g/L+agar 5.8 g/L(pH 5.8) and MS+NAA1.0 mg/L+sucrose 30 g/L+agar 5.8 g/L(pH 5.8) respectively. The callus induction rate was Yushu4＞Yushu 7＞Yi 306＞ Nongda 22 in four sweet potato varieties and the callus induction rate was petiole＞ leaf＞ stem tip＞ stem segment in different explants. Microtubers were not obtained in the microtuber test.