栀子黄色素的提取及纯化工艺的优化

摘要

为获得高品质的栀子黄色素,采用微波辅助法,并通过比较5种大孔树脂(AB-8,HPD-100A,D101,HPD-80,X-5)的纯化条件和效果,对栀子黄色素的提取及其产品纯化工艺进行了优化.结果表明:微波提取的最佳工艺组合为A2B1C1D3,即提取功率600 W,乙醇浓度30％,提取时间3 min,料液比1∶8,此条件下产品的色价为81.66,OD值为1.25;经纯化试验,HPD-100A型大孔树脂适宜纯化栀子黄色素,精制后得到的产品质量较高,色价达489,OD值为0.27.%For getting high quality gardenia yellow, the extraction and purification technology of gardenia yellow was optimized through comparing the purification effects of five kinds of macroporous resins(AR-8, HPD-100A, D101 , HPD-80 and X-5) by microwave assisted extraction method. The results showed that the best combination was A2 B1 C1 D3, extraction power 600 W, ethanol concentration 30%, extraction time 3 min, solid-liquid ratio 1:8. Under these conditions, the color value and OD value were 81. 66 and 1. 25 respectively. The purification test showed that the macroporous resin HPD-100A was suitable for purifying gardenia yellow for its high color value(489) and OD value(0. 27).