抑制茶饼病菌放线菌及细菌的筛选与鉴定

摘要

The antagonism actinomycete and bacteria strains against E.vexans were screened from tea rhizoaphere soil by plate dilution and spraying methods and then identified by ITS sequence analysis based on the morphological characteristics to solve pesticides residues in control of tea blister blight.The results showed that antagonism actinomycete C6 strain with 27.00 mm of average inhibition zone and bacterium B-9 strain with 32.00 mm of average inhibition zone is screened from 59 actinomycete strains and 19 bacterium strains isolated from tea rhizoaphere soil respectively.The antagonism actinomycete C6 strain and bacterium B-9 strain is identified as Streptomyces acidiscabies and Bacillus subtilis respectively.%为筛选出用于生产上防治茶饼病的生防菌，克服化学农药防治造成的农药残留问题，采用平板稀释和喷雾法，从茶树根际土壤中筛选抑制茶饼病菌的拮抗放线菌和细菌，结合形态学特征与 ITS 序列测定的方法对拮抗菌进行鉴定。结果表明：从采集土样中共分离到59株放线菌和19株细菌，从中各筛选出1株对茶饼病菌具有拮抗作用的放线菌（C6）和细菌（B-9），平均抑菌圈直径分别为27．00 mm 和32．00 mm；经鉴定，C6为酸疮痂链霉菌（Streptomyces acidiscabies ），B-9为枯草芽孢杆菌（Bacillus subtilis ）。