高温对早籼稻花后剑叶氮代谢关键酶活性及籽粒蛋白质含量的影响

摘要

为探讨高温对不同类型早籼稻开花后剑叶中氮代谢关键酶活性及籽粒蛋白质含量的影响,利用人工气候室在籽粒灌浆成熟期进行高温(日均温31.5 ℃)和适温(日均温23.5 ℃)处理,对2个早籼稻品种(湘早籼24和株两优611)灌浆成熟期籽粒中蛋白质含量及剑叶中GS和GOGAT活性的动态变化进行了分析.结果表明:供试2个品种在高温下花后剑叶中GS和GOGAT活性先升后降,均在开花后10 d出现峰值;适温下花后剑叶中GS和GOGAT活性呈缓慢下降趋势;花后饲用稻湘早籼24号各时期剑叶中GS和GOGAT活性均比食用稻株两优611的高.高温条件下湘早籼24号花后22 d,1 g干重籽粒中的蛋白质含量比适温条件下的增加4.75 mg,株两优611增加1.88 mg.说明籽粒成熟期高温有助于提高早籼稻剑叶中GS和GOGAT活性及籽粒中蛋白质含量,更有利于高蛋白饲用稻剑叶GS和GOGAT活性及籽粒中蛋白质含量的提高.%Estigate the effects of high temperature on key enzymes(GS and GOGAT)activity of nitrogen metabolism in flag leaf and protein content after flowering in two different early indica rice, high temperature(31.5 C)and optimal temperature(23.5 ℃)are treated to two different type early indica rice(Xiangzaoxian 24 and Zhuliangyou 611).The results showed that the GS and GOGAT activity were highest on 10 d under high temperature treatment and were slowly decreased under optimal temperature treatment. Activity of two kinds of key enzyme in Xiangzaoxian 24 were both higher than that in Zhuliangyou 611. Compared to optimal temperature treatment in Xiangzaoxian 24 under high temperature,the protein content was 4.75 mg higher on 22 d and 1.88mg higher for Zhuliangyou 611. These results suggest that high temperature can help to improve two early indica rice(especial the Xiangzaoxian 24)the GS and GOGAT activity in flag leaf and grain protein content.