拟南芥成花调控LFY基因的选择性剪接

摘要

To study the expression of flowering regulatory gene LFY gene in Arabidopsis thaliana,we used reversetranscription-polymerase chain reaction and isolated three alternative splicing(AS)fragments named as LFY1239,LFY1263,and LFY1275 respectively.Sequence analysis confirmed that fragment LFY1263 contained an open reading　frame of 1 263 bp,and was identical to the previously reported and predicted fragment,while LFY1239 lacked 36bp at　the 3' end of the first exon,and LFY1275 had an additional 12bp derived from the 3' end of the first intron.Expression analysis showed that LFY1239 was only detected in rosette during the vegetative stage,while LFY1263 and　LFY1275 presented in both floral organs and rosette during both vegetative and flowering stages.Furthermore,LFY1263 appeared to be the most abundant transcript.The expression ratio of LFY1275 to LFY1263 was higher in　floral organs than in rosette leaves,which suggested that such ratio might be associated with the flowering regulation.%为研究拟南芥成花调控基因LFY,我们采用RT-PCR方法分离克隆了三种选择性剪接的片段,分别命名为LFY1239,LFY1263和LFY1275.序列分析表明LFY1263包含一个大小为1 263 bp的开放阅读框,与之前报道的LFY基因片段大小相同,而LFY1239在第一外显子的3'端缺失了36 bp,LFY1275在第一内含子的3'末端插入了12 bp.对几种片段表达部位的分析显示,LFY1239只能在营养生长期的莲座叶中表达,而LFY1263和LFY1275在营养生长期和花期的花器官和莲座叶中都可以检测到,并且,LFY1263呈现出主导地位,LFY1275与LFY1263表达的比例表现为花器官高于莲座叶,该比例的变化可能预示着与成花调控有关.