罗汉果祛痰作用谱效关系研究

摘要

In order to investigate the relationship between expectorant efficacy and active components of Siraitia grosvenorii, the phenol red secretion of trachea in mice and the HPLC fingerprint of S.grosvenorii extract were determined.Grey relational analysis was applied to confirm the contribution degree of each common peak from HPLC fingerprints for expectoration efficacy, while partial least-squares regression (PLS) was utilized to confirm either positive or negative relationship, and to identify the contribution degree of S.grosvenorii extract.The results indicated that fourteen common peaks were chosen in HPLC fingerprints, including twelve peaks with relation rate higher than 0.8 (No.3, 11, 12, 13 peaks were positive correlation peaks, and No.1, 2, 4-10, 14 peaks were negative correlation peaks), No.11 and 12 peaks were Oxomogroside V and Mogroside V respectively.Therefore, expectorant efficacy of S.grosvenorii is contributed by a combined action of multi-components rather than one component.Oxomogroside V and Mogroside V have an expectorant efficacy and high contribution degree.%为了研究罗汉果祛痰作用与活性成分的相关性,该研究首先测定了19批罗汉果提取物高效液相(HPLC)指纹图谱和小鼠气管酚红排泌量,找出了指纹图谱共有峰,采用灰色关联度法确定各共有峰对祛痰效果贡献度,利用偏最小二乘法(PLS)得出了各共有峰的正负相关及贡献度.结果表明:共有峰为14个,其中关联度在0.8以上的有12个峰(正相关峰为3、11、12、13号峰,负相关峰为1、2、4-10、14号峰);11、12号峰分别为氧化罗汉果苷Ⅴ和罗汉果苷Ⅴ.因此,罗汉果祛痰作用不是单一成分起作用,而是多种成分综合作用的结果.其中,氧化罗汉果苷Ⅴ和罗汉果苷Ⅴ有祛痰作用,贡献率较大.