PAI-1对卵巢癌上皮细胞SKOV3生物学行为的影响

摘要

To investigate the impact of plasminogen activator receptor-l( PAI-1 )gene on the biological behavior of ovarian cancer cells SKOV3 in vivo and in vitro. Methods The PAI-1 gene was transfected into SKOV3 cells. The cell cycle was detected by cell growth curve, colony formation test and flow cytometry assay. The function of PAI-1 in SKVO3 cells were investigated by detecting the capability of invasion,migration,adhesion. Results PAI-1 gene could be successfully transfected and expressed steadily. The PAI-1 protein could be detected by Western blot in target cells. The assay results of cell cycle and colony formation showed that PAI-1 enhanced cell proliferation of SKOV3 significantly. In addition, PAI-1 was upregulated in SKOV3 cells could also contribute to the ability of cell invasion,migration and adhesion. Conclusion PAI-1 can enhance the ability of cell proliferation,invasion, migration and adhesion in ovarian cancer cells SKOV3. Therefore,PAI-1 may be plays a dual role in tumor cell invasion and metastasis.%目的 探讨PAI-1基因在体外实验中对卵巢癌细胞SKOV3生物学行为的影响.方法 将PAI-1基因转到入人卵巢癌细胞SKOV3中,通过细胞生长曲线、细胞克隆形成实验、流式细胞仪检测细胞周期,测定细胞侵袭迁移黏附能力,研究PAI-1基因在卵巢癌上皮细胞SKOV3中的功能.结果 PAI-1基因被转入靶细胞中并稳定表达,Western blot能检测到PAI-1基因蛋白在靶细胞中的表达;在细胞生长周期的测定和克隆形成实验中,转导入PAI-1基因后的细胞(SKOV3-PAI-1)较未转导PAI-1基因的SKOV3细胞增殖能力明显增强;经流式细胞仪检测,转导PAI-1基因的SKOV3细胞增殖能力亦明显增强;PAI-1基因的表达显著增强了SKOV3细胞的体外侵袭迁移黏附能力.结论 PAI-1的表达增强了SKOV3卵巢癌上皮细胞增殖和体外侵袭、迁移及黏附能力,PAI-1可能在肿瘤细胞侵袭和转移中起着双重作用.