脂蛋白脂酶基因Ser447stop多态性与脑卒中的关系

摘要

目的 探讨脑卒中与脂蛋白脂酶基因Ser447stop多态性的相关性.方法 脑梗死患者87例,脑出血患者71例,对照组85例,采用聚合酶链反应和限制性片段长度多态性方法(PCR-RFLP法)检测脂蛋白脂酶基因Ser447stop多态性,测定血脂水平.结果 对照组Ser447stop C等位基因为80.0%,G等位基因为20.0%;脑梗死组分别为93.1%、6.9%,脑出血组分别为90.14%、9.86%;脑梗死组、脑出血患者组G等位基因检出率较对照组低(P＜0.05),CC基因型检出率高于对照组(P＜0.05),CG基因型显著低于对照组(P＜0.01).脑梗死、脑出血患者组血清三酰甘油(TG)、LDL-c水平较对照组高(P＜0.01);脑梗死、脑出血患者组血清Apo AⅠ、ApoB水平较对照组低(P＜0.01).结论 脂蛋白脂酶基因Ser447stop多态性与脑卒中有一定关联,LPL Ser447stopG等位基因突变是脑卒中发病的一个保护性因素.%Objective To study the association of lipoprotein lipase Ser447stop gene polymorphisms with cerebral infarction and cerebral hemorrhage. Methods The lipoprotein lipase Ser447stop genotypes were detected by PCR-RFLP in 227 subjects( including 87 cerebral infarction patients and 71 cerebral hemorrhage patients and 85 controls ). The serum levels of lipids and apolipoproteins were detected in all the subjects. Results The C allele in control group was 81.18％,G allele 18.82％ ;the C allele cerebral infarction group was 93.1％ ,G allele 6. 9％ ;the C allele in cerebral hemorrhage group was 93.1％ ,G allele 6.9％ ;the ratio of G allele was significantly different among the three groups( P ＜0.05 );The TG,LDL-c levels in cerebral infarction group and cerebral hemorrhage group were higher than that in the control group ( P ＜0.05 );and the ApoAI,ApoB100 levels in cerebral infarction group and cerebral hemorrhage group were lower than that in the control group( P＜0.01 ). Conclusion Lipoprotein lipase Ser447stop gene polymorphism is correlated with cerebral infarefion,LPL Ser447stop G allele mutation is a protective factor for cerebral infarction and cerebral hemorrhage.