Graves病患者ANA、ENA谱检测的临床意义

摘要

Objective To exptore the ctinicat significance of ANA and ENA profite in diagnosis of Graves disease. Methods ANA was measured by indirect immunoftuoreseenee,ENA profite was measured by Eurontine immunobtot assay. Resutts In the 140 samptes, poskives deteetion rate of ANA and ENA profite were 27.87% and 26.43% , respectivety, which were higher than those of the controts( 1.4% ,0,P ＜0.01). ANA pattems were mainty of spot type and homogeneous-nucteotar in patients with (Graves disease. ENA profite resuks indicated that the antinuetear antibodies were mainty anti-nRNP/Sm, anti-SSA,and anti-SSB. Conctusion Combined determination of ANA and ENA profite coutd confirm the types of auto antibodies in the serum of patients with (Graves disease, which coutd provide reference for diagnosis and treatment of Graves disease.%目的 探讨Graves病患者抗核抗体(ANA)和核抗原抗体(ENA)谱的临床应用价值.方法 ANA检测采用间接免疫荧光法,ENA谱检测采用欧蒙印迹法.结果 140例Graves病患者中,ANA及ENA谱阳性率分别为27.87%和26.43%,均高于对照组的1.4%及0%(P＜0.01).Graves病患者中ANA阳性时的荧光模式主要是斑点型、核均质型,ENA谱阳性抗体种类出现较多的是抗nRNP/Sm抗体、抗SSA抗体、抗SSB抗体等.结论 检测Graves病患者ANA及ENA谱,能明确Graves病患者血清中的自身抗体种类,是Graves病的诊断和疗效观察的参考指标.