宫颈癌患者血清中血管内皮生长因子C和D及其受体的表达及临床意义

摘要

目的 检测血管内皮生长因子C和D(VEGF-C、VEGF-D)及其受体血管内皮生长因子受体3(VEGFR-3)在宫颈癌血清中的表达,并探讨VEGF-C、VEGF-D和VEGFR-3与宫颈癌淋巴转移机制相关性.方法 应用酶联免疫分析法(ELISA)检测正常宫颈组10例,宫颈上皮内瘤变CINⅢ组36例,宫颈癌无淋巴结转移患者组33例,宫颈癌有淋巴结转移患者组10例,检测血清中VEGF-C、VEGF-D、VEGFR-3的浓度表达并比较各组间差异性.结果 (1)宫颈癌无淋巴结转移者组VEGF-C、VEGF-D、VEGFR-3血清水平均高于正常宫颈组及CINⅢ组,差异有统计学意义(P＜0.05);(2)宫颈癌有淋巴结转移者组VEGF-C、VEGF-D、VEGFR-3血清水平均高于宫颈癌无淋巴结转移组,差异有统计学意义(P＜0.05);(3)CINⅢ组VEGF-C、VEGF-D、VEGFR-3血清水平与正常宫颈组相比,差异无统计学无意义(P＞0.05).结论 宫颈癌患者血清VEGF-C、VEGF-D 和VEGFR-3的表达水平均与淋巴结转移相关,且VEGF-C、VEGF-D 与VEGFR-3的血清表达水平密切相关,所以VEGF-C、VEGF-D可能在宫颈癌中通过VEGFR-3信号通道促进淋巴结转移.其可能成为推测宫颈癌淋巴结转移和预后的有用的生物学指标.%Objective To detect vascular endothelial growth factor C and D( VEGF-C and VEGF-D ) and their receptor-3( VEGFR-3 ) levels in serum of patients with cervical cancer, and to explore the correlation of lymph node metastasis with VEGF-C, VEGF-D and VEGFR-3. Methods ELISA was used to detect the expression levels of VEGF-C, VEGF-D and VEGFR-3 in serum of 10 cases of normal cervical tissues,36 cases of cervical intraepithelial neoplasia Ⅲ( CINⅢ group ),33 cases of cervical cancer without lymph node metastasis, 10 cases of cervical cancer with lymph node metastasis. The difference of expression levels of VEGF-C, VEGF-D and VEGFR-3 were compared among different groups. Results ( 1 )The expression level of VEGF-C, VEGF-D and VEGFR-3 in serum of cervical cancer patients without lymph node metastasis was significantly higher than those in normal cervical tissues group and CIN1 group( P <0.05 );( 2 )The expression level of VEGF-C, VEGF-D and VEGFR-3 in serum of cervical cancer patients with lymph node metastasis was significantly higher than that in cervical cancer patients without lymph node metastasis( P <0. 05 );( 3 )There was no significant difference in expression levels of serum VEGF-C, VEGF-D and VEGFR-3 between CINⅢ group and normal cervical tissues group ( P >0. 05 ). Conclusion The expression levels of VEGF-C,VEGF-D and VEGFR-3 are related to lymph node metastasis in patients with cervical cancer,and the expression levels of VEGF-C and VEGF-D are closely related to that of VEGFR-3. Thus, VEGF-C and VEGF-D may increase the incidence of lymph node metastasis in cervical cancer through VEGFR-3 signaling pathway, which may be used as the valuable indicators for predicting lymph node metastasis and prognosis of cervical cancer.