miRNA-135 a在阿尔茨海默病患者血液标本及PC-12细胞乏外吐小体中的变化及临床意义

摘要

Objective To study the changes and clinical significance of miRNA-135a in blood samples and exosome of PC-12 cells from patients with Alzheimer′s disease.Methods Nine patients with mild cognitive impairment (MCI),21 patients with dementia of Alzheimer type ( DAT ) and 30 healthy controls were detected for the expression level of miRNA-135a in serum,plasma and medium.Results The relative expression level of miRNA-135a in exosome medium was lower than that in ordinary medium(P<0.05).In the healthy control group,the relative expression level of miRNA-135a in plasma was higher than that in serum ,which was also higher than that in exosome medium (P<0.05).The relative expression level of serum or plasma miRNA-135a in the MCI group or DAT group was lower than that in the control group (P<0.05).Conclusion The expression level of miRNA-135a is low in patients with cognitive impairment ,the detection of miRNA-135 a is valuable in the clinical diagnosis of Alzheimer′s disease .%目的：探讨miRNA-135 a在阿尔茨海默病患者血液标本及PC-12细胞乏外吐小体中的变化及临床意义。方法选取轻度认知障碍患者（ MCI）9例，痴呆期阿尔茨海默病（ DAT）患者21例，健康对照者30例，检测受试者血清、血浆及培养基中miRNA-135 a表达水平。结果在乏外吐小体培养基中miRNA-135 a相对表达量低于普通培养基（P＜0．05）；健康对照组血浆中miRNA-135a相对表达量高于血清，也高于乏外吐小体培养基（ P＜0．05）；MCI组、DAT组血清、血浆中miRNA-135 a相对表达量均低于对照组（ P＜0．05）。结论认知障碍患者血miRNA-135a表达量低，检测miRNA-135a对临床诊断阿尔茨海默病有一定价值。