苦参碱对体外培养人增生性瘢痕成纤维细胞 MMP-1、MMP-9表达的影响

摘要

Objective To study the effects of matrine on the expressions of matrix metalloproteinase (MMP)-1, MMP-9 in human hypertropic scar fibroblasts ( HSFb) in vitro.Methods HSFb was cultured in vitro by being treated with different concentrations of matrine[0 mmol/L(control group),2.5 mmol/L,5.0 mmol/L,10.0 mmol/L].Enzyme-linked immunoadsorbent assay(ELISA) was used to detect the expressions of MMP-1,MMP-9 in the treated supernatants.Results The expression levels of MMP-1,MMP-9 in groups of different concentrations of matrine were higher than those in the control group 24 or 48 hours after matrine treatment(P<0.05),the expression levels of MMP-1,MMP-9 in the 5.0 mmol/L matrine group were higher than those in the 2.5,10.0 mmol/L matrine groups ( P<0.05).Conclusion Matrine can increase the expression of MMP-1,MMP-9.It can be applied to hyperplastic scar prevention and treatment .%目的：探讨苦参碱对体外培养人增生性瘢痕成纤维细胞（ HSFb ）基质金属蛋白酶（ MMP-1、MMP-9）表达的影响。方法将浓度分别为0 mmol／L（对照组）、2．5 mmol／L、5．0 mmol／L、10．0 mmol／L苦参碱作用于体外培养人HSFb，采用酶联免疫吸附试验检测加药后上清液中MMP-1、MMP-9表达量。结果苦参碱作用24 h、48 h时各浓度组MMP-1、MMP-9的表达量均高于对照组（P＜0．05），5．0 mmol／L组表达量明显高于2．5 mmol／L组、10．0 mmol／L组（ P＜0．05）。结论苦参碱可增加HSFb MMP-1、MMP-9的表达，抗瘢痕作用好，在增生性瘢痕防治上具有一定的应用前景。