来氟米特对脂多糖诱导下 HaCaT细胞分泌 IL-8和 ICAM-1的影响

摘要

Objective To observe the effects of leflunomide on the secretion of interleukin 8(IL-8) and intercellular adhesion molecule 1(ICAM-1) in the lipopolysaccharide-induced HaCaT cell.Methods Lipopolysaccharide was used to induce the proliferation of HaCaT cell ,supernatants were harvested to detect the levels of IL-8 and ICAM-1 by ELISA.Results The levels of IL-8 and ICAM-1 increased after induction of 1μg/ml lipopolysaccharide,and decreased after treatment with different concentrations of leflunomide(the concentrations ranged from 30 μg/ml to 270 μg/ml),which was concentration-dependent. Conclusion Leflunomide can inhibit the secretion of IL-8 and ICAM-1 in the lipopolysaccharide-induced HaCaT cell.%目的：观察来氟米特活性代谢产物A771726对脂多糖诱导下人永生化角质细胞（ HaCaT ）分泌白介素-8（IL-8）和细胞间黏附分子-1（ICAM-1）的影响。方法用脂多糖诱导HaCaT细胞增殖，ELISA法检测细胞上清液中IL-8、ICAM-1的含量。结果1μg/ml的脂多糖可以诱导HaCaT细胞高分泌IL-8和ICAM-1，加入A771726（浓度范围为30～270μg/ml）后能够抑制其分泌，抑制作用随浓度的增大而增强。结论来氟米特可以抑制脂多糖诱导下HaCaT细胞分泌IL-8、ICAM-1。