咯利普兰对大鼠脊髓横断损伤后神经元凋亡的影响

摘要

目的：探索咯利普兰对大鼠脊髓横断损伤后神经元凋亡的影响。方法取成年SD大鼠60只分为假手术组（ Sham组）、损伤组（ SCI组）及咯利普兰治疗组（ R组）各20只。在R组及SCI组建立大鼠脊髓横断损伤模型，Sham组大鼠仅打开椎板；R组术后立刻皮下注射咯利普兰，SCI组及Sham组皮下注射等体积二甲基亚砜。于术后6 h、12 h、24 h、72 h检测各组脊髓半胱氨酸天门冬氨酸蛋白酶3（caspase-3）及B淋巴细胞瘤蛋白2（Bcl-2）的表达水平，并计算各组脊髓的脱氧核苷酸末端转移酶介导的dUTP缺口末端标记（ TUNEL）染色阳性细胞数量。结果术后各时间点，R组的Bcl-2表达水平高于SCI组及Sham组（P＜0．05），R组的caspase-3表达水平低于SCI组（P＜0．05）；术后12 h、24 h、72 h，R组TUNEL阳性细胞数量少于SCI组（P＜0．05）。结论咯利普兰可明显减少大鼠脊髓横断损伤后神经元的凋亡。%Objective To investigate the effect of rolipram on neuronal apoptosis after spinal cord transverse injury in rats .Methods A total of 60 adult SD rats were divided into sham-operation group(Sham group,n=20),spinal cord injury group(SCI group,n=20) and rolipram treatment group(Group R,n=20).The rat model of spinal cord transverse injury was established in SCI group and Group R ,and the rats in Sham group were only disposed of opening the lamina .Group R was injected subcutaneously with rolipram immediately after operation , and SCI group and Sham group were injected subcutaneously with the same volume of dimethyl sulfoxide .The expression levels of spinal cord caspase-3 and Bcl-2 were detected in each group on the 6th,12th,24th,and 72nd hour after operation.The amounts of [terminal deoxynucle-otidyl transferase(TdT)-mediated dUTP nick end labeling,TUNEL] positive cells in spinal cord were counted in each group .Results At each time point after operation , the expression level of Bcl-2 in Group R was significantly higher than that in SCI group or Sham group (P<0.05),and the expression level of caspase-3 in Group R was lower than that in SCI group(P<0.05).On the 12th,24th,and 72nd hour after operation,the number of TUNEL positive cells in Group R was significantly lower than that in SCI group (P<0.05).Conclusion Rolipram can significantly reduce the neuronal apoptosis after spinal cord transverse injury in rats .