目的探索咯利普兰治疗大鼠脊髓横断损伤的可行性。方法取健康成年雌性Sprague-Dawley大鼠30只，随机分为假手术组(Sham组)、损伤组(SCI组)、咯利普兰治疗组(R组)，每组10只。R组建立大鼠脊髓横断损伤模型后立刻皮下注射咯利普兰；Sham组仅打开椎板；SCI组及Sham组皮下注射相同体积二甲基亚砜(DMSO)。于损伤后2、4、6、8周采用BBB评分法观察大鼠后肢运动功能情况，损伤后2周时应用免疫组织化学染色检测各组生长相关蛋白-43(GAP-43)及胶质细胞酸性蛋白(GFAP)的表达。结果损伤后6周、8周时，R组BBB评分优于SCI组(P<0.05)。损伤后2周，R组GAP-43表达高于SCI组(P<0.05)，GFAP表达量低于SCI组(P<0.05)。结论咯利普兰能提高大鼠脊髓横断损伤神经功能评分，提高GAP-43表达，抑制GFAP表达。%Objective To investigate the possibility of rolipram for treatment of spinal cord injury (SCI) in rats. Methods 30 adult fe-male Sprague-Dawley rats were divided into sham-operation group (sham group, n=10), spinal cord injury group (SCI group, n=10) and ro-lipram treatment group (R group, n=10). The rats in SCI group and R group were modeled as spinal cord transection injury, and R group was administrated with rolipram subcutaneouly after SCI. They were assessed with Basso Beattie and Bresnahan (BBB) score 2, 4, 6, and 8 weeks after SCI, and the expressions of growth associated protein 43 (GAP-43) and glial fibrillary acidic protein (GFAP) were detected with immunohistochemistry 2 weeks after SCI. Results There were significant difference in the BBB scores between SCI and R groups 6 and 8 weeks after SCI (P<0.05). The expression of GAP-43 was more and GFAP was less in R group than in SCI group (P<0.05). Conclusion Ro-lipram can increase the expression of GAP-43 and inhibit the expression of GFAP, while improves the the motor function in rats after spinal cord transsection injury.
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