乳脂肪球表皮生长因子8蛋白在原发性乳腺癌中的表达及其临床意义

摘要

Objective To explore the expression of milk fat globule epidermal growth factor 8(MFG-E8) in primary breast cancer and its clinical significance.Methods Immunohistochemistry EnVision method was used to detect the expressions of MFG-E8 in 74 cases of primary breast cancer tissues without distant metastasis( non-metastasis group) and their matched adjacent normal breast tissues,and 55 cases of breast cancer tissues with distant metastasis(metastasis group).The relationship between positive rate of MFG-E8 and clinicpatological characteristics was analyzed.The expressions of progesterone receptor,estrogen receptor and human epidermal growth factor receptor-2(HER2) were detected in non-metastasis group.The correlation between positive rate of MFG-E8 and expressions of three receptors was analyzed.Results The positive rates of MFG-E8 in the breast cancer tissues without distant metastasis,the adjacent normal breast tissues and breast cancer tissues with distant metastasis were 85.1%(63/74),91.9%(68/74),92.7%(51/55),respectively.There was no significant difference in the positive rate of MFG-E8 among three groups(P>0.05).The positive rate of MFG-E8 in non-metastasis group increased with the decreasing histological grade(P<0.05).The positive rate of MFG-E8 in non-metastasis group positively correlated with the expression of estrogen receptor(P <0.05),but didn′t significantly correlate with the expressions of progesterone receptor and HER2 (P>0.05). Conclusion Overexpression of MFG-E8 is observed in non-metastatic and metastatic breast cancer tissues,which might play a certain role in the assessment of prognosis as well as the guidance of clinical treatment for breast cancer.%目的 探讨乳脂肪球表皮生长因子8蛋白( MFG-E8)在原发性乳腺癌组织中的表达情况及其临床意义. 方法 采用免疫组化EnVision二步法检测MFG-E8在74例未发生远处转移的原发性乳腺癌组织(未转移组)及其癌旁正常乳腺组织、55例发生远处转移(转移组)的乳腺癌组织中的表达情况,分析MFG-E8阳性率与各临床病理特征的关系;检测74例未转移组乳腺癌组织雌激素受体、孕激素受体和人类表皮生长因子受体2(HER2)的表达情况,分析其与MFG-E8阳性率的相关性. 结果 未转移组乳腺癌组织、癌旁正常乳腺组织、转移组乳腺癌组织MFG-E8阳性率分别为85.1%(63/74)、91.9%(68/74)、92.7%(51/55),3组MFG-E8阳性率比较,差异无统计学意义(P>0.05). 未转移组MFG-E8阳性率随组织学分级降低而升高(P<0.05);未转移组乳腺癌组织MFG-E8阳性率与雌激素受体表达呈正相关(P<0.05),与孕激素受体、HER2均无明显相关性(P>0.05). 结论 MFG-E8在未转移和转移乳腺癌组织中均高表达,其对判断乳腺癌预后、指导治疗有一定作用.