俞原配穴针刺对慢性心肌缺血大鼠的保护作用及其机制研究

摘要

Objective To investigate the protective effects of combined acupuncture at transport and source acupoints on rats with chronic myocardial ischemia and its mechanism .Methods Forty-eight Wistar rats were randomly divided into control group ,model group , combined acupoint group and single acupoint group ,with 12 cases in each group .The chronic myocardial ischemia model was developed by injection with isoprenaline hydrochloride in each group except the control group .After the successful modeling , the rats in the combined acupoint group were acupunctured at Shenmen ,Daling,Xinshu and Queyinshu acupoints ,while the rats in the single acupoint group were only acupunctured at Neiguan acupoint .And no intervention was performed in the control group or the model group .The cardiac function indexes including left ventricular systolic pressure ( LVSP ) , left ventricular end-diastolic pressure ( LVEDP ) , maximum velocity of left ventricular pressure rise( +dp/dtmax),maximum velocity of left ventricular pressure fall ( -dp/dtmax),and heart rate were compared among all groups.The changes in the levels of serum superoxide dismutase (SOD),malonaldehyde(MDA),lipid peroxidation(LPO) and glutathione peroxidase(GSH-PX),as well as myocardial infarct sizes were observed in each group .Results Compared to the model group,LVSP,+dp/dtmax ,-dp/dtmax ,heart rate,SOD and GSH-PX levels were higher while MDA and LPO levels and LVEDP were lower in the combined acupoint group(all P<0.05) after 15 days of intervention.Compared to the single acupoint group,LVSP,+dp/dtmax ,-dp/dtmax , SOD and GSH-PX levels were higher while LPO level was lower in the combined acupoint group(all P<0.05).The myocardial infarct size in the combined acupoint group was less than that in the model group or the single acupoint group(all P<0.05).Conclusion Combined acupuncture at transport and source acupoints has protective effects on rats with chronic myocardial ischemia ,and its mechanism may correlate with the role of the combined acupuncture in alleviating lipid peroxidation damage and reducing the severity of myocardial injury .%目的 探讨俞原配穴针刺对慢性心肌缺血大鼠的保护作用及其机制.方法 48只Wistar大鼠随机分成对照组、模型组、配穴组、单穴组各12只,除对照组外每组大鼠注射盐酸异丙肾上腺素建立慢性心肌缺血模型.造模成功后,配穴组给予针刺神门、大陵、心俞、厥阴俞穴,单穴组仅针刺内关穴,对照组与模型组不作处理.比较各组大鼠左心室收缩压(LVSP)、左心室舒张末期压(LVEDP)、左室压上升最大速率(+dp/dtmax)、左室压下降最大速率(-dp/dtmax)、心率等心功能指标,观察各组大鼠血清超氧化物歧化酶(SOD)、丙二醛(MDA)、脂质过氧化物(LPO)、谷胱甘肽过氧化物酶(GSH-PX)水平及心肌梗死面积的变化.结果 干预15 d后,与模型组比较,配穴组大鼠的LVSP、+dp/dtmax、-dp/dtmax、心率、SOD、GSH-PX水平较高,MDA、LPO、LVEDP较低(均P<0.05);与单穴组比较,配穴组LVSP、+dp/dtmax、-dp/dtmax SOD、GSH-PX水平较高,LPO水平较低(均P<0.05);配穴组心肌梗死面积小于模型组及单穴组(均P<0.05).结论 俞原配穴针刺对慢性心肌缺血大鼠具有保护作用,其作用机制可能与俞原配穴针刺降低脂质过氧化损伤、减轻心肌受损程度有关.