色素框同源蛋白8在大肠癌组织中的表达及其临床意义

摘要

目的 探讨色素框同源蛋白8(CBX8)在大肠癌组织及癌旁组织中的表达情况及其意义.方法 应用荧光定量实时聚合酶链反应(qRT-PCR)和免疫组化方法检测172例大肠癌患者的癌组织、癌旁组织中CBX8的表达情况,并分析其与临床病理因素的关系.根据大肠癌组织CBX8的表达水平将172例患者分为CBX8低表达组70例、高表达组102例.结果 大肠癌组织中CBX8表达水平及阳性率均明显高于癌旁组织(P＜0.05);CBX8低表达组、高表达组患者的性别、年龄、诊断(直肠癌或者结肠癌)比较,差异无统计学意义(P＞0.05);CBX8低表达组、高表达组患者肿瘤细胞分化程度、术前血清癌胚抗原(CEA)水平、是否血管侵犯及TNM分期比较,差异有统计学意义(P＜0.05);Cox单因素分析显示,肿瘤细胞分化、术前血清CEA水平、血管侵犯以及癌组织CBX8的表达是影响大肠癌患者预后的危险因素(P＜0.05);Cox多因素分析显示,术前血清CEA水平、血管侵犯、癌组织CBX8表达是影响大肠癌患者预后的独立因素(P＜0.05).生存分析显示,两组1年生存率比较,差异无统计学意义(P＞0.05),但CBX8高表达组患者3年、5年生存率均明显高于低表达组(P＜0.05).结论 大肠癌组织中CBX8呈高表达,然而癌组织中CBX8高表达提示患者预后良好.%Objective To investigate the expression and significance of chromobox protein homolog 8 (CBX8) in colorectal cancer and adjacent tissues.Methods Quantificational real-time polymerase chain reaction(qRT-PCR) and immunohistochemistry were used to detect the expression of CBX8 in tumor and adjacent tissues among 172 patients with colorectal cancer,and the relationship between the CBX8 expression and clinicopathological parameters was analyzed.One hundred and seventy-two patients were divided into low-expression CBX8 group(n =70) and high-expression CBX8 group(n =102) depending on the expression level of CBX8 in colorectal cancer tissues.Results The expression level and positive rate of CBX8 in colorectal cancer tissues were significantly higher than those in the adjacent tissues (P ＜ 0.05).There was no significant difference in gender,age or diagnosis (rectal cancer or colon cancer) between low-expression and high-expression CBX8 groups (P ＞ 0.05).There were significant difference in the differentiation of tumor cell,preoperative serum level of carcinoembryonic antigen(CEA),vascular invasion and TNM stage between low-expression and high-expression CBX8 groups (P ＜ 0.05).Cox univariate analysis revealed that differentiation of tumor cell,preoperative serum CEA level,vascular invasion,CBX8 expression of tumor tissues were independent factors of prognosis influencing colorectal cancer(P ＜ 0.05).Survival analysis showed that there was no significant difference in the 1-year survival rate between the two groups (P ＞ 0.05),however,the 3-and 5-year survival rates in the high-expression CBX8 group were significantly higher than those in the low-expression CBX8 group (P ＜ 0.05).Conclusion The expression of CBX8 in colorectal cancer tissues is high,and shows a good prognosis.