增液八珍汤对大鼠胃泌素、P物质及生长抑素含量的影响∗

摘要

目的：观察增液八珍汤对便秘大鼠血液及肠壁组织中胃泌素( GAS)、P物质( SP)及生长抑素( SS)含量的影响，揭示其治疗便秘的药效机理。方法将120只大鼠随机分为正常组20只，模型组100只给予甘草煎剂灌胃7天，将模型组100只大鼠随机分为模型组、增液八珍汤低、中、高剂量组及西沙必利组各20只。分别灌胃给药7天后，检测各组大鼠血液及肠壁组织中GAS、SP、SS的含量。结果增液八珍汤低、中剂量组与模型组比较三项指标均有显著差异( P<0．05)，高剂量组差异更明显( P<0．01)；增液八珍汤低、中剂量组与西沙必利组比较三项指标均有显著差异(P<0．05)，高剂量组差异更明显(P<0．01)。结论增液八珍汤可通过调节血液及肠壁组织中GAS、SP、SS的含量，达到促胃肠动力的作用。其中增液八珍汤高剂量组疗效显著，明显优于西药对照组。%Objective To observe the effect of Zengye Bazhen decoction on gastrin ( GAS ) , substance P ( SP ) and somatostatin ( SS ) content in blood and intestinal tissue of rats, and reveal its pharmacodynamic mechanism. Methods 120 rats were randomly divided into normal group with 20 rats and model group with 100 rats. The rats in the model group were given gastric lavage Licorice decoction for 7 days, and then they were randomly divided into model group, low, middle and high dose of Zengye Bazhen decoction and cisapride group, with 20 rats in each group. Each group was given gastric lavage for 7 days. The content of GAS, SP and SS in blood and intestinal tissue of rats were tested. Results There were significant differences between low or middle dose of Zengye Bazhen decoction groups and the model group (P<0. 05), and the high dose group had more significant difference (P<0. 01). There were significant differences between low or middle dose of Zengye Bazhen decoction groups and the cisapride group (P<0. 05), and the high dose group had more significant difference (P<0. 01). Conclusion Zengye Bazhen decoction can adjust the content of GAS, SP and SS in blood and intestinal tissue of rats, and increase the force of intestinal propelling. The high dose of Zengye Bazhen decoction group had more excellent effect, and was superior to the Western medicine group.