Objective: To explore the change of 5-hydroxytryptamine (5-HT), somatostatin (SS), substance P (SP) immu noreactive (IR) cells in the rat ileum during heroin dependence period. Methods: Normal SD rats were divided into a normal control group (NCG), a saline control group (SCG) and a heroin dependence group (HDG). The heroin dependence model of rats was made by subcutaneous injection of herion. Immunohistochemical SABC method, counting the number of cells and image analysis were used in the study. Results: Heroin dependence caused a significant increase in the number of 5-HT im munoreactive cells in the ileum, not NCG and SCG cells. And, the mean grey degree of 5-HT, SS and SP immunoreactive products in the ileum was significantly lower than that in the control and saline groups. Conclusion: The changes indicate that the expression of 5-HT, SS and SP increase in the rat ileum during heroin dependence.%目的:探讨海洛因依赖对大鼠回肠5-羟色胺(5-HT)、生长抑素(SS)和P物质(SP)免疫反应(IR)细胞的影响.方法:选取成年SD大鼠,分为正常对照组、盐水对照组和海洛因依赖组,建立大鼠海洛因依赖模型,取回肠组织用免疫组织化学SABC法及图像分析法进行研究.结果:与正常及盐水对照组比较,海洛因依赖组大鼠回肠5-HT免疫反应细胞增多,SS及SP免疫反应细胞数无明显变化.5-HT、SS-、SP-IR细胞免疫显色增强,图像分析显示海洛因依赖期间大鼠回肠内5-HT、SS及SP-IR细胞的平均灰度值均低于正常及盐水对照组.结论:海洛因依赖期间,回肠5-HT、SS和SP-IR细胞的5-HT、SS和SP表达增加.
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