增强表达PTEN蛋白对类风湿关节炎成纤维样滑膜细胞增殖的作用

摘要

目的 研究增强表达PTEN蛋白对类风湿关节炎成纤维样滑膜细胞(RA FLS)增殖的影响.方法 慢病毒表达载体pLenti6/V5-PTEN转染RA FLS并表达PTEN蛋白,应用RT-PCR、Western blot检测PTEN表达,MTT法检测RA FLS增殖能力的变化.结果 转染pLenti6/V5-PTEN后,RA FLS中PTEN mRNA表达提高60%以上,PTEN蛋白表达在48 h后提高了107.85%(P＜0.05),RA FLS增殖在48、72、96 h分别下降了25.14%、41.84%和52.46%(P＜0.05).结论 慢病毒表达载体pLenti6/V5-PTEN能够提高PTEN蛋白表达水平,并显著抑制RA FLS增殖.%Objective To study the effects of PTEN expression on the reproduction of FLS in rheumatoid arthritis ( RA ). Methods FLS were transfected with pLenti6/V5-PTEN for over-expression of PTEN. Subsequently, the expression of PTEN was assessed with RT-PCR and Western Blot. The proliferation of FLS was also assessed. Results After transfected with pLenti6/V5 - PTEN, the expression of PTEN mRNA and protein in FLS were significantly increased by 63% and 107. 85% , respectively ( P <0. 05 ). At the same time the proliferation of FLS was significantly reduced by 25. 14% , 41. 84% and 52. 46% at 48 h, 72 h and 96 h, respectively. Conclusion Lentivirus vector pLenti6/V5-PTEN can enhance PTEN expression, thus effectively inhibits FLS proliferation in RA.