VacA+和CagA+的幽门螺杆菌重复序列基因分型研究

摘要

Objective To investigate the correlation between Helicobacter pylori ( Hp ) with VacA + or CagA + and repetitive sequence genotype. Methods The amplification of VacA and CagA gene fragments were conducted with PCR. Strains were genotyped with REP - PCR and further clustered with NTsys_2 software. Results The 26 VacA and CagA gene positive strains were divided into six genotype groups according to homology, both with 3,3,8,4,6,2 strains in each Hp group, respectively. Conclusion The VacA and CagA positive strains could be divided into six genotype groups, and the repetitive sequence genotype is not associated with VacA and CagA gene.%目的 探索VacA+和CagA+与幽门螺杆菌(Hp)重复序列基因分型的关系.方法 采用PCR方法确定VacA+或CagA+ Hp菌株,重复序列基因分型方法分别对26株VacA+和CagA+的菌株进行基因分型,并运用NTsys_2软件,根据相似性78%进行聚类分型.结果 VacA+和CagA+的26株Hp均被分为6个基因型,分别是Group Ⅰ、Group Ⅱ、Group Ⅲ、Group Ⅳ、Group Ⅴ和Group Ⅵ,且每类聚集的菌株数相同,分别是3、3、8、4、6、2株.结论 VacA+和CagA+的Hp可以分成6大类基因型,VacA+和CagA+与Hp重复序列基因分型无密切关系.