血管生成相关因子在口腔扁平苔藓中的表达

摘要

目的 研究血管生成相关因子血管内皮生长因子(vascular endothelial growth factor,VEGF)、细胞间黏附分子-1(intercellular adhesion molecule-1,ICAM-1)、血管细胞黏附分子-1(vascular cell adhesion molecule-1,VCAM-1)在口腔扁平苔藓患者血清和组织中的表达情况.方法 30例扁平苔藓患者纳入扁平苔藓组,15例健康志愿者作为对照(正常组)用于血清收集,收集扁平苔藓组织患者活检组织30例及外科手术切除的多余正常口腔黏膜组织15例,应用免疫组织化学技术分析VEGF、ICAM-1、VCAM-1分别在扁平苔藓病损局部组织及正常黏膜组织中的表达情况.结果 ELISA结果显示VEGF在2组血清中表达差异无统计学意义(t=1.837,P=0.074),而ICAM-1(t=2.730,P=0.009)、VCAM-1(t=2.167,P=0.035)在扁平苔藓组血清中的表达明显高于正常组.免疫组织化学染色结果显示VEGF、ICAM-1、VCAM-1在口腔黏膜正常上皮中呈现少量阳性表达或阴性表达,而在扁平苔藓组织中VEGF(x2=33.632,P<0.05)、ICAM-1(x2=45.000,P<0.05)、VCAM-1(x2=37.286,P<0.05)表达较正常黏膜组织均出现上调趋势.结论 在扁平苔藓患者的血清和病变组织中,血管生成相关因子表达上调.%Objective To detect the expression of vascular endothelial growth factor (VEGF), intercellular adhesion molecule-1 (ICAM-1), vascular cell adhesion molecule-1 (VCAM-1) in oral lichen planus (OLP). Methods Thirty OLP patients and fifteen healthy subjects were enrolled in the study. Serum were collected from 15 healthy volunteers as con-trols. Normal tissues were collected from surgical department as immunohistochemical analysis. The levels of VEGF, ICAM-1, VCAM-1 in serum were measured by ELISA. Immunohistochemical analysis of VEGF, ICAM-1, VCAM-1 were carried out by the means of primary antibodies and anti-VEGF, anti-CD106 antigen (VCAM-1) and anti-CD54 anti-gen (ICAM-1). Results ELISA results showed no expression differences for VEGF between the two groups. Whereas, the levels of ICAM-1, VCAM-1 in OLP group were significantly higher than those in control group (P<0.05). Immuno-histochemical results reveal the presence of a significant angiogenesis in OLP patients through the immunoexpression of VEGF, ICAM-1, VCAM-1 according to the percentage of stained cells (P<0.05). Conclusion Regarding the results, it seems that high expression of VCAM-1 and ICAM-1 are related to oral lichen planus.