豆制品中碱性橙Ⅱ、酸性橙Ⅱ和碱性嫩黄O的测定

摘要

High performance liquid chromatography(HPLC) with UV detector method was developed tbr the analysis of Basic Orange Ⅱ, Acid Orange Ⅱ and Auramine O in bean products. Samples was extracted by acetonitrile and water(7+3), and determined by HPLC with UV detector. The calibration curve of the method was linear over the range of 0.1 to 20.0 μg/mL with a correlation coefficient of 0.9999. The recoveries for Basic Orange Ⅱ, Acid Orange Ⅱ and Auramine O standard spiked in the range of 5.0 to 15.0 mg/kg were between 88.8 % to 93.0 % with a relative standard deviation (RSD) being between 0.67 % to 1.66 %(n=6). The detection limit was Basic Orange 11 : 0.02 mg/kg, Acid Orange Ⅱ: 0.05 mg/kg, Auramine O: 0.05 mg/kg. Owing to advantages of good linearity, high precision and rapidity, this method is suitable FOr the determination of Basic Orange Ⅱ, Acid Orange Ⅱ and Auramine O in bean products.%建立了高效液相色谱紫外检测器测定疆制品中碱性橙Ⅱ、酸性橙Ⅱ和碱性嫩黄O的方法。样品片]乙腑-水（7＋3）溶液提取，经高效液相色谱仪紫外榆测器测定。结果表明：碱性橙Ⅱ、酸性橙Ⅱ和碱性嫩黄O在O．1-20μg／mL范围内呈良好线性关系．相关系数均为0．9999，3个水平（5．0、10．0、15．0mg／kg））加标回收率为88．8％～93．0％，相对标准偏差为O．67％-1．66％（n：6），检出限分别为碱性橙Ⅱ：0．02mg／kg、酸性橙II：O．05mg／kg和碱性嫩黄0：0．05mg／kg。此力‘法具有简单、快速、准确等优点，适用于甄制品中碱性橙Ⅱ、酸性橙Ⅱ和碱性嫩黄O的同时测定。