不同禾本科牧草种子萌发及幼苗耐盐性鉴定

摘要

The relative germination rate,seed germination potential,embryo root and embryonic bud ratio, germination index,simplified vigor index of 5 grass species were measured and analyzed by the method of Petri dishes of paper germination and pot seedling cultivation under 7 different mixed salt solutions of NaCl and Na2 SO4 (0.4% to 1.6%).MDA and free proline contents were measured and compared in another 5 different mixed salt solutions (0.3%~1.5%).The effect of salt stress on the seed germination and seedling growth were evaluated and analyzed by membership function to provide theoretical reference for salt tolerance identification. The results showed that the order of salt tolerance of 5 grass species was:Elymus nutans >Agropyron trachy-caulum ,Elymus sibiricus > Agropyron cristatum > Bromus inermis .%采用培养皿纸上发芽法和盆栽幼苗培育法,在 NaCl 和 Na2 SO4复盐溶液7个盐浓度梯度下(0.4%~1.6%),比较分析了5种禾本科牧草种子的相对发芽率、相对发芽势、胚根胚芽比、发芽指数、简化活力指数；在 NaCl 和 Na2 SO4复盐溶液5个盐浓度梯度下(0.3%~1.5%),比较分析了5种禾本科牧草幼苗的 MDA 含量和游离脯氨酸含量的变化。运用隶属函数评价、分析盐胁迫对不同禾本科牧草种子萌发及幼苗的影响,为牧草耐盐性鉴定、耐盐育种和盐碱地牧草栽培提供理论参考。研究结果表明,5种禾本科牧草在萌发期和苗期耐盐能力排序依次为：垂穗披碱草>细茎冰草、老芒麦>扁穗冰草>无芒雀麦。