通心络对早期糖尿病肾病大鼠肾功能及肾组织NO/eNOS表达的影响

摘要

Objective To investigate the influence of Tong Xinluo on renal function and the ex-pression of No, eNOS in kidney, and explore its mechanism of action. Methods The rat model was estab-lished by high fat feed and injection of streptozotocin. The model rats were separated into 3 groups random-ly:the model group(n=10), the valsartan group(n=10) and Tong Xinluo group(n=10). Another 10 normal rats were chosen as the normal group. Chinese herb medicine group was given Tong Xinluo 0. 4 g/( kg·d) and Western medicine group was given valsartan 10 mg/( kg·d) for 8 weeks. Fasting blood glu-cose(FBG), Urinary albumin excretion(UAER) , serum creatinine(Scr), blood urea nitrogen(BUN), Creatinine clearance rate( Ccr) were examined and calculated in 4weeks and 8weeks. After 8 weeks, all the rats were sacrificed, weighing their renal and observing the expression of NO, eNOS in kidney. Differ-ences among groups were assessed by one-way anova. Results Compared with normal group, the FBG, UAER , Scr , BUN, Ccr, kidney weight and relative kidney weight in model group are significantly in-creased(P<0. 05). Comperd with model group, the expression of FBG in valsartan group and Tong Xinluo group did not change(P>0. 05), while the UAER, Scr , BUN, Ccr, kidney weight and relative kidney weight significantly decreased(P<0. 05). The expression of NO, eNOS of TongXinluo group and valsartan group is also decreased(P<0. 05). Conclusion Tong Xinluo can decrease the expression of UAER, Scr , BUN, Ccr in DN rats, and then protect renal function. Decreasing the expression of NO and eNOS and then reducing glomerular hyperfiltration would be one of its action mechanism.%目的：观察通心络对高脂饲料联合小剂量链脲佐菌素( streptozotocin, STZ)诱导的早期糖尿病肾病( diabetic nephropathy, DN)大鼠血清一氧化氮( nitric oxid, NO)含量及内皮型一氧化氮合酶( eNOS)蛋白表达的影响,探讨通心络防治早期DN大鼠肾小球高滤过的作用机制。方法采用高脂饲料联合小剂量STZ建立DN大鼠模型,将DN大鼠随机分为模型组、缬沙坦组、通心络组各10只,设立10只正常SD大鼠为空白组,通心络组给予通心络超微粉0．4 g/( kg·d),缬沙坦组给予缬沙坦10 mg/( kg·d)干预8周,于治疗4周、8周监测空腹血糖( fasting blood glucose, FBG),尿微量白蛋白排泄率( urinary albumin excretion, UAER )、血肌酐( serum creatinine, SCr )及尿素氮( blood urea nitrogen, BUN),并计算内生肌酐清除率( creatinine clearance rate, Ccr)。于8周末处死大鼠,称量肾重,计算肾重指数,检测肾组织NO含量及eNOS蛋白表达。多组组间差异比较采用单因素方差分析。结果与空白组比较,模型组大鼠FBG、UAER、肾重及肾重指数、Scr、BUN、Ccr、NO含量显著升高、eNOS蛋白表达显著增强( P<0．05)。与模型组比较,通心络组及缬沙坦组FBG无明显变化(P>0．05),UAER、Scr、BUN、Ccr、肾重及肾重指数显著下降(P<0．05)。通心络组及缬沙坦组肾组织NO含量较模型组显著降低,eNOS蛋白表达较模型组减弱(P<0．05)。结论通心络可降低早期DN大鼠UAER、Scr、BUN、Ccr、肾重及肾重指数,保护肾功能。通心络抑制肾组织eNOS蛋白表达,减少肾组织NO的含量,从而降低早期DN大鼠肾小球率过滤,可能是通心络改善肾小球早期高滤过的作用机制之一。