Objective To observe the effect of Tongxinluo on vascular endothelial growth factor ( VEGF) expression in rats with early stage of diabetic nephropathy .Methods The rat model of type 2 di-abetes mellitus was induced by injection of low-dose of streptozocin after having high-fat diet for 4 weeks, 40 rats were randomly divided into 4 groups-normal group, diabetic group, valsartan treated group(10mg/kg/d) and Tongxinluo treated group (0.4mg/kg/d);After 8 weeks, the level of urinary albumin excretion rate (UAER), creatinine clearance rate (Ccr),kidney weight/body weight ratio (KW/BW) and plasma VEGF was measured and the renal expression of VEGF and Flt 1 was determined by western blot and real-time PCR.Results The level of UAER, Ccr, KW/BW, VEGF an Flt1 expression in Tongxinluo treated group was lower than it in diabetic group (P<0.05),while FBG had no significant difference .Conclusion Tongxinluo can improve glomerular hyperfiltration and albuminuria excretion in rats with early stage of di -abetic nephropathy;The mechanism could be related to inhibition of VEGF and Flt 1 expression .%目的：观察通心络对早期糖尿病肾病大鼠血管内皮生长因子表达的影响。方法采用高脂饲料+小剂量链脲佐菌素建立2型糖尿病大鼠模型，随机分为空白组10只、模型组10只、缬沙坦组10只（10mg／kg／d）和通心络组10只（0．4mg／kg／d）；治疗8周后，比较各组大鼠的空腹血糖、尿白蛋白排泄率、内生肌酐清除率及肾重指数，采用酶联免疫吸附试验测定血浆血管内皮生长因子含量，western blot及RT－PCR测定肾组织中血管内皮生长因子及其受体Flt1的表达。结果模型组大鼠空腹血糖、尿白蛋白排泄率、内生肌酐清除率、肾重指数、血浆血管内皮生长因子含量、肾组织血管内皮生长因子及Flt1表达较空白组有不同程度的升高（P＜0．05），通心络组除空腹血糖外各指标水平较模型组均显著降低（ P＜0．05）。结论通心络可以有效改善早期糖尿病肾病大鼠的高滤过状态，抑制尿蛋白的排出，其作用机制与抑制血管内皮生长因子及其受体Flt1的过度表达有关。
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