基于显微-DNA 条形码-产地分析联用的巴西柑橘属植物药 FARINHA DE LARANJA（枳壳）基原鉴定研究

摘要

Objective o study the variety identification of a kind of herbal powder of FARINHA DE LARANJA of Citrus L. in Brazil with a combaned analysis of microscope - DNA barcoding - origin. Methods Microscopic identification was used to identify the variety of samples preliminarily; then ITS se-quences were amplified by PCR and sequenced, and BLAST in NCBI database was used to calculate the similarity of sequences to identify variety of samples. , Finally, the DNA barcoding identification results were screened and analyzed according to its geographical origin information. Results The combined analysis of microscopic - DNA barcoding - origin indicated that FARINHA DE LARANJA collected from market in Brazil was the dried fruit powder of Citrus aurantium. Conclusion The identification of Brazil FARINHA DE LARANJA could enrich the alternative resources of Zhiqiao and Zhishi.%目的：利用显微-DNA 条形码-产地分析联用的方法对巴西柑橘属植物药 FARINHA DE LARANJA 粉末进行基原鉴定研究。方法应用显微鉴定的方法对药材粉末的基原进行初步判定；再提取样品 DNA,扩增 ITS 序列,利用 NCBI 数据库中的 BLAST 功能分析相似度,对样品品种进行DNA 条形码鉴定；最后结合产地信息对数据库中的序列进行筛选。结果显微-DNA 条形码-产地综合分析结果表明,该样品为芸香科柑橘属 Citrus L.植物酸橙 Citrus aurantium 的干燥未成熟果实粉末。结论巴西植物药 FARINHA DE LARANJA 的鉴定可为扩大枳实、枳壳等中药材的药用资源提供依据。