助仙丹对多囊卵巢模型大鼠卵巢功能及形态的影响

摘要

目的：观察助仙丹对多囊卵巢大鼠模型卵巢形态和性激素的影响。方法将60只大鼠随机分为2组,空白对照8只,另52只大鼠采用避孕硅胶棒联合皮下往射人绒毛膜促性腺激素方法进行造模,连续监测两个动情周期,选出造模成功大鼠30只,随机分为三组：助仙丹组(10只﹚、炔雌醇环丙孕酮片组(10只﹚、模型组(10只﹚,称重,记为用药前大鼠体质量,助仙丹组、炔雌醇环丙孕酮片组分别给予助仙丹配方颗粒和炔雌醇环丙孕酮片灌胃,模型组及空白对照组给予等量自来水灌胃,14天后再次称重,记为用药后体质量,后腹主动脉取血,检测血清雄激素浓度,并摘除双侧卵巢。利用单因素方差分析的统计学方法,观察各组大鼠用药前后体质量变化,卵巢质量变化,及血清雄激素浓度变化,并观察闭锁卵泡数量、颗粒细胞层数目,卵泡发育状态等卵巢形态变化。结果与模型组比较,炔雌醇环丙孕酮片组大鼠治闻前后体质量增加值明显降低,有显著差异(P<0.05﹚,炔雌醇环丙孕酮片组大鼠卵巢质量与模型组比较,有显著差异( P<0.05﹚；各组大鼠雄激素水平未见明显差异。助仙丹颗粒配方组大鼠卵巢切片与模型组大鼠相比：闭锁卵泡数量减少,卵巢颗粒细胞层数明显增加,可见正常发育阶段卵泡及成熟黄体组织。结论助仙丹可改善多囊卵巢综合征大鼠卵巢形态、促进正常排卵。%Objective To investigate the effect of traditiona1 Chinese medicine modified Zhuxian Dan( TCM-ZXD﹚ on ovary tissues patho1ogy and improving sex hormone in rat mode1s of po1ycystic ovary syndrome( PCOS﹚. Methods 60 rats were random1y divided into 2 groups, 52 rats in patho1ogica1 mode1 group and 8 in norma1 contro1 group. Patho1ogica1 mode1 group was subcutaneous imp1anted of progesterone ge1 rod combined with HCG for bui1ding PCOS mode1s. According to the vagina1 smear, 30 successfu1 mode1 rats were se1ected and then random1y divided into three subgroups:TCM-ZXD group ( 10 rats﹚, Diane-35 group (10 rats﹚, mode1 group (10 rats﹚. A11 the rats were weighed, recorded as body weight before treatment. TCM-ZXD group and Diane-35 group were given the right amount of formu1a granu1es of TCM-ZXD and Diane-35, whi1e the mode1 group and the contro1 group were given the same amount of water. After 14 days of therapy, the rats were weighed again, recorded as body weight after treatment. n B1ood was co11ected from abdomina1 aorta, then T in serum was determined. The weight of the ovary was recorded and ovarian morpho1ogy was observed. The resu1ts were ana1yzed by one-way anova. Result Compared with the mode1 group, the added va1ue of body weight of Diane-35 group before and after the treatment decreased significant1y ( P<0. 05﹚, there was a significant change in the Diane-35 group of the rats ovarian weight (P<0. 05﹚. There was no obvious difference in androgen 1eve1s in rats between these groups. According to the Ovary paraffin, TCM-ZXD group cou1d increase the 1ayers of ovarian granu1osa ce11s and the number of 1utea1 tissue, reduce the number of 1ocked fo11ic1es. Conclusion Zhuxian Dan can improve the ovarian morpho1ogy and promote ovu1ation in rats with PCOS.