电针“百会”、“涌泉”两穴对 APP/PS1双转基因小鼠学习记忆行为与海马超微结构的影响

摘要

Objective To observe the effect of electro-acupuncture( EA) on the abilities of spatial learning andmemory and the hippocampus ultrastructure in APP/PS1 double transgenic mice. Methods APP/PS1 double transgenic mice (4-month-old) are divided into model group, EA group and drug(AChE) group according to the random assignment method, age-matched one brood mice with negative transgenes were used as control group, with 12 mice in each group. For the EA group, EA was applied to Baihui (GV20) and Yongquan ( KI 1) for 15 min, once every other day for 5 weeks. For the drug group, 0. 92 ml/g of acetylcholine enzyme was given by intragastricadministration. The learning-memory ability and space exploration ability of the mice were tested by using Morris water maze. Remove the brains to make and observe the hippocampal CA 1 area transmission electric lens. Results The escape latency periods of the mice were detected by Morris water maze for four days, the result showed that there was a statistical difference between the model group and the control group (P<0. 05); space exploration experiment re-vealed that the activity time of the model group in the original platform quadrant ( the third quadrant) is sig-nificantly lower than that of the control group(P<0. 05);transmission electron microscopy results showed that the control group is superior to the model group in micro-vessels, neuron synapses and ultrastructure of neurons. Conclusion EA therapy can improve the learning-memory ability of the APP/PS1 double trans-genic mice and positively regulate the hippocampus ultrastructure, which might be one of the mechanisms for EA to improve the learning-memory behavior of AD mice.%目的观察电针对淀粉样前蛋白/早老蛋白1(amyloid precursor protein/presenilin-1, APP/PS1)双转基因鼠空间学习记忆行为及海马超微结构的影响。方法将4月龄的APP/PS1双转基因小鼠按随机分配法分为模型组、电针组和药物组,以相同月龄同窝阴性小鼠作为对照组,每组12只。电针组取“百会”、“涌泉”两穴,每次留针时间为15分钟,隔日1次,针刺5周；药物组予乙酰胆碱酶抑制剂0．92 mL/g灌胃。 Morris水迷宫检测小鼠学习记忆能力及空间探索能力,取脑做海马CA1区透射电镜片并观察。结果 Morris水迷宫检测四天小鼠上台潜伏期结果显示：模型组与对照组比较有统计学差异(P<0．05)；空间探索实验：模型组在原有平台象限(第三象限)区域活动时间明显低于对照组(P<0．05)；透射电镜结果显示：在微血管、神经元突触及神经元超微结构上对照组优于模型组。结论电针治疗可改善APP/PS1双转基因小鼠学习记忆能力,并对海马超微结构有良性调节作用,这些表现可能是电针改善AD小鼠学习记忆行为学的机制之一。