慢性浅表性胃炎不同证型大鼠琥珀酸脱氢酶活性与细胞色素C含量对比研究

摘要

目的：探讨慢性浅表性胃炎( chronic superficial gastritis, CSG)脾虚与湿热证模型大鼠胃组织琥珀酸脱氢酶( succinate dehydrogenase,SDH)的活性及细胞色素C( cytochrome C,Cyt-C)的含量变化,从能量代谢的角度揭示CSG不同证候的实质。方法用水杨酸钠溶液灌胃法复制大鼠单纯CSG模型,在此基础上,用小承气汤泻下法及饥饱失常法复制脾虚CSG模型；用肥甘辛辣饮食法复制湿热CSG模型,5周后,检测大鼠胃组织琥珀酸脱氢酶活性及细胞色素C含量。结果 SDH的含量为：湿热CSG组最高,而脾虚CSG组最低。脾虚CSG组与湿热CSG组分别与其他组比较,差异均有统计学意义(P<0．05或P<0．01)；胞浆中Cyt-C含量造模组均高于正常组,差异有统计学意义(P<0．05或P<0．01),其中脾虚CSG组最高,湿热CSG组次之,两组之间比较,差异有统计学意义(P<0．01)。结论 CSG不同证型大鼠胃组织SDH活性及Cyt-C含量的差异,提示同一疾病不同证型之间能量代谢状态的不同,湿热证相对于脾虚证能量代谢较亢进。%Objective To explore the mechanisms of different syndromes in terms of the energy metabolism by observing the activity changes of succinate dehydrogenase ( SDH) and the content changes of cytochrome C ( Cyt-C) in rats with chronic superficial gastritis ( CSG) of spleen-deficiency syndrome and damp-heat syndrome. Methods CSG rat model was established by the intragastric administration of sodi-um salicylate solution. CSG rat model with spleen-deficiency syndrome was established by Xiaochengqi De-coction-induced purgation and eating disorders. CSG rat model with damp-heat syndrome was established by feeding fat, sweet and spicy diet. Five weeks later, SDH activity and Cyt-C content of gastric tissue were determined. Results The activity of SDH in damp-heat CSG group was the highest of all, but which in spleen-deficiency CSG group was the lowest . The activity of SDH was obviously changed in damp-heat CSG group also in spleen-deficiency CSG group in comparison with the other three groups, respectively. ( P<0. 05 or P<0. 01). The content of Cyt-C in the three model groups was higher than which in normal group (P<0. 05 or P<0. 01). The content of Cyt-C in spleen-deficiency CSG group was the highest of all, with the damp-heat CSG group secondarily. There was a significantly content difference between the two groups (P<0. 01) . Conclusion The difference in the SDH activity and Cyt-C content of CSG rat with different syndromes , revealed the different energy metabolism of different syndromes for the same disease, the ener-gy metabolism of damp-heat symdrome was higher than which of spleen-deficiency syndrome.