酸角壳提取物对蔗糖负荷大鼠血糖及α-葡萄糖苷酶和α-淀粉酶的影响

摘要

目的 研究酸角壳提取物对蔗糖负荷大鼠糖吸收的抑制作用.方法 采用蔗糖负荷大鼠模型观察酸角壳提取物对蔗糖负荷后不同时间点血糖的影响;采用酶标法测定酸角壳提取物对正常大鼠肠黏膜和内容物中α-葡萄糖苷酶和α-淀粉酶活性的影响,评价酸角壳提取物对肠道α-葡萄糖苷酶和α-淀粉酶活性的影响.结果 各剂量酸角壳提取物能显著降低蔗糖负荷后15分钟大鼠的血糖水平(P＜0.01).酸角壳提取物能明显抑制小肠上段和中段α-葡萄糖苷酶以及小肠中段α-淀粉酶的活性,在给药30分钟到1小时的抑制作用最为显著.结论 酸角壳提取物能够显著降低蔗糖负荷后大鼠的血糖水平,其作用可能与抑制肠道α-葡萄糖苷酶和α-淀粉酶活性有关.%Objective To study the inhibitory effects of tamarind shell extract on intestinal absorption of sucrose.Methods The sucrose load model of rats were used and blood glucose levels were analyzed chronologically to evaluate the effect of tamarind extract.In vivo experiments were also performed to study the inhibitory effects of tamarind shell extract on the activities of α-glucosidase and α-amylase in the small intestinal mucosa and contents of rats.Results Tamarind shell extract of all doses had significant effects on the blood glucose levels after 15 min and 30 min of surcrose administration (P＜0.01).Tamarind shell extract also significantly inhibited the activities of α-glucosidase and α-amylase in the small intestinal mucosa and contents.Conculusion Tamarind shell extract could effectively reduce the blood glucose level in sucrose-loaded rats.The mechanism might be related to its inhibitory effects on the activities of α-glucosidase and α-amylase.