益气化瘀解毒方治疗内毒素致 ARDS大鼠的炎症作用研究

摘要

目的：探讨益气化瘀解毒方(以下简称“复方”)对内毒素( lipopolysaccharide,LPS)诱发大鼠急性呼吸窘迫综合征( acute respiratory distress syndrome,ARDS)的保护作用及对炎症因子的影响。方法50只雄性健康SD大鼠随机等分为5组,每组10只。5组大鼠按动物饲养标准进行适应性喂养1周。中药低、中、高剂量组,分别给予复方低、中、高剂量灌胃,每天1次,模型组及空白对照组每天予蒸馏水灌胃,每天灌胃量为1 mL/100 g,除空白对照组外其余4组7天后尾静脉注射LPS (2 mg/kg)造模,空白对照组及造模4组16小时麻醉下处死。取肺组织及腹主动脉血,进行大鼠肺大体病理、病理组织光镜观察,并检测血清中肿瘤坏死因子α( tumor necrosis factor alpha,TNF-α)、白细胞介素1β( interleukin 1beta,IL-1β)、IL-6及肺组织中p65蛋白含量。结果与空白对照组比较,模型组TNF-α升高(P<0.05)。与模型组比较,低剂量组大鼠血清中TNF-α无明显降低(P>0.05),中药中、高剂量TNF-α水平显著降低(P<0.05),但高、中剂量组之间无明显差距(P>0.05)。与空白对照组比较,模型组IL-1β升高(P<0.05)；与模型组比较,低剂量组IL-1β降低(P<0.05),中与高剂量组IL-1β水平明显降低(P<0.05)。与空白对照组比较,模型组IL-6升高(P<0.05)；与模型组比较,低剂量组IL-6明显降低(P<0.05),中剂量组IL-6水平显著降低(P<0.05),高剂量组IL-6水平明显降低(P<0.05)。与空白对照组比较,模型组p65升高(P<0.05)；与模型组比较,低剂量组p65明显降低(P<0.05),中剂量组p65水平显著降低(P<0.05),中药高剂量组p65水平降低(P<0.05)。结论益气化瘀解毒复方能够减轻内毒素致ARDS大鼠肺组织损伤,具有一定的预防保护作用,可能与降低大鼠血清中TNF-α、IL-1β、IL-6水平,抑制NF-κB通路激活,减轻炎症反应有关。在一定范围内,提高复方的给药剂量,可以增强作用效果。%Objective To study protective effect of Yiqi Huayu Jiedu recipe on acute respiratorydistress syndrome ( ARDS) rats induced by lipopolysaccharide and to observe the influence of in-flammatory factor. Methods 50 healthy male SD rats were randomly divided into 5 groups(n=10). All rats were fed for one week to adapt the environment. The rats were intragastric administration with corresponding drug. The ARDS model was injected with LPS (2mg/kg) after 7 days. The rats were sacrificed after 16 hours of modeling. Lung tissue was collected to observed the pathology organization, and the TNF-α, IL-1β, IL-6, p65 protein in serum were detected. Results TNF- alpha: compared with the blank control group, serum TNF-αof rats in the model group was increased(P<0. 05). Compared with the model group, TNF-α of low dose of Chinese medicine group was decreased non-significantly, TNF-α of medium and high dose of Chinese medicine group was decreased significantly (P<0. 01), but there was no significant between two groups; Compared with the blank control group, IL-1, IL-6 and p65 protein of model group rats was increased(P<0. 05). Compared with the model group, IL-1, IL-6 and p65 protein of Chinese medicine groups were decreased (P<0. 05). Conclusion Yiqi Huayu Jiedu recipe can reduce the lung tissue injuries of ARDS rats induced by LPS, and can reduce the level of TNF-α, IL-1β, IL-6, can inhibit the NF-κB pathway activation and reduce inflammation reactions. In a certain range, improve the dosage of compound, and enhance the effect.